Catalog # EK6220

EphA4 Receptor Activation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

The Eph family of Receptor tyrosine kinases and their Ephrin ligands are important for cell positioning and morphogenesis during development. Eph receptors are classified into 10 EphA and 6 EphB receptors, which preferentially bind to the type A and type B ephrins, respectively. The EphA4 receptor can inhibit axon outgrowth and has roles in regulating axon projections during neural development. EphA4 signaling pathways require its kinase activity and involve binding and activation of Rho-GTPase guanine nucleotide-exchange factors (GEFs). EphA4 activation leads autophosphorylation of Tyr-596 and Tyr-602, and the conserved sites in EphA2 are required for binding to the GEFs, Vav2 and Vav3, and ephrin-induced cell migration. The Tyr-779 site in the kinase domain is also phosphorylated in vivo and may regulate kinase activity. Activated EphA4 leads to Src kinase phosphorylation of the GEF, ephexin-1, and this activates RhoA. Thus, EphA4 signaling involves complex tyrosine phosphorylation in its cytoplasmic region along with interaction with several GEFs.

References
Binns, K.L. et al. (2000) Mol. Cell. Biol. 20(13):4791.
Lackmann, M. & Boyd, A.W. (2008). Sci. Signal. 1(15):re2.
Fang, W.B. et al. (2008) J. Biol. Chem. 283(23):16017.

Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).

Paraformaldehyde-fixed zebrafish embryos were probed with anti-EphA4 (Tyr-602) (EP2731) then detected using Alexa Fluor 647 goat anti-rabbit. Arrows show labeling of somite boundaries and the notochord. (Image provided by Dr. Scott Holley at the Department of Molecular, Cellular and Developmental Biology, Yale University.)



Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

EM2801 Spanevello, M. et al. (2013) J Neurotrauma. 30(12):1024. WB : mouse EphA4
EP2731 Defourny, J. et al. (2013) Nat Commun. 4:1438. ICC: mouse cochlea and growth cone
EP2731 Park, E. et al. (2013) Cell Death Differ. 20(1):169. ICC: mouse embryo
EP2731 Spanevello, M. et al. (2013) J Neurotrauma. 30(12):1024. WB : mouse EphA4
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
EP2731 Ortalli, A.L. et al. (2012) PLoS ONE. 7(6):e38566. ICC: Chicken embryo ganglion neurons
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
EP2731 Julich, D. et al. (2009) Development 136:2913. IHC: zebrafish embryos

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
EphA4 (C-terminal region) Rabbit pAb50 μlWB, EHu, Rt, Ms120
EphA4 (N-terminal region) Mouse mAb50 μlWB, EHu, Rt, Ms, Ck120
EphA4 (Tyr-602)[conserved site], phospho-specific Rabbit pAb 50 μlWB, E, IHCHu, Rt, Ms, Ck120
EphA4 (Tyr-779)[conserved site], phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck120
Anti-Mouse Ig:HRP Donkey pAb 100 μlWB, EMs
Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb 100 μlWB, E, ICC, IHCRb
KIT SUMMARY

The EphA4 activation antibody sampler kit can be used to detect EphA4 phosphorylation of Tyr-602 and Tyr-779. The kit also includes rabbit polyclonal and mouse monoclonal antibodies to examine total EphA4 expression levels and secondary reagents for rabbit polyclonal and mouse monoclonal antibody detection.