Catalog # DK6690

DISC1 Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution
WB, AB, E, ICC, IHC

Species Reactivity

Rt, Ms, Rb

$395


DATASHEET  

KIT SUMMARY

The DISC1 phospho-regulation antibody kit can be used to detect phosphorylation of Ser-710 relative to total DISC1 expression. The kit includes two rabbit polyclonal antibodies to detect total expression of DISC1 and secondary reagents for detection of rabbit polyclonal antibody in western blot or ELISA. In addition, the kit includes peptides for blocking experiments or use as ELISA positive controls.

Western blot of DISC1 phosphorylation in PC12 cells untreated (lanes 1 & 3) or treated with calyculin A (100 nm) for 30 min (lanes 2 & 4). The blots were probed with DP3041 anti-DISC1 (a.a. 740-753) (lanes 1 & 2) or DP3061 anti-DISC1 (Ser-710) (lanes 3 & 4).

Western blot of DISC1 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with DP3021 anti-DISC1 (a.a. 740-753) (lanes 1 & 2) and DP3041 anti-DISC1 (a.a. 740-753) (lanes 3 & 4).



Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide, and the peptides are supplied in PBS and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

DP3041 Kumar, S. et al. (2015) Neurosci Res. 97:1 WB: PC12 cells
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

CATALOG #DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
DISC1 (a.a. 740-753) Rabbit pAb50 μlWB, ERt, Ms100
DISC1 (a.a. 740-753), Rabbit pAb50 μlWB, ERt, Ms100
DISC1 (Ser-710), phospho-specific Rabbit pAb 50 μlWB, ERt, Ms100
DISC1 (a.a. 740-753) Peptide50 μg AB, E
phospho-DISC1 (Ser-710) Peptide50 μgAB, E
Anti-Rabbit Ig Light Chain Specific:HRP Mouse mAb 100 μlWB, E, ICC, IHCRb