Disrupted in schizophrenia 1 (DISC1) is a multifunctional scaffold protein that has important roles in neurodevelopment. DISC1 is expressed in both neuronal progenitor cells and postmitotic neurons in the developing cerebral cortex. DISC1 can interact with both centrosomal proteins and dynein-motor related proteins. In addition, DISC1 interacts with and inhibits the kinase GSK-3β to enable Wnt activation of β-catenin–mediated gene transcription and neuron proliferation. These functions of DISC1 may be regulated by post-translational modification. PKA and CDK5 can phosphorylate Ser-710, and a non-phosphorylatable Ser-710 mutant shows decreased interaction with the centrosomal proteins, BBS1 and BBS4, while a constituitively phosphorylated Ser-710 mutant shows increased interaction with these proteins. During neuronal progenitor cell proliferation, DISC1 phosphorylation at Ser-710 is low and the interaction with GSK-3β is enhanced. By contrast, DISC1 phosphorylation is increased, association with GSK-3β is decreased, and interaction with BBS1 is enhanced during postmitotic neuron migration.
Brandon, N.J. et al. (2011) Nat Rev Neurosci. 12(12):707.
Ishizuka, K. (2011) Nature. 473(7345):92.
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:DP3041 Kumar, S. et al. (2015) Neurosci Res. 97:1 WB: PC12 cells
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
|CATALOG #||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||MW (kDa)|
|DISC1 (a.a. 740-753) Rabbit pAb||50 μl||WB, E||Rt, Ms||100|
|DISC1 (a.a. 740-753), Rabbit pAb||50 μl||WB, E||Rt, Ms||100|
|DISC1 (Ser-710), phospho-specific Rabbit pAb||50 μl||WB, E||Rt, Ms||100|
|DISC1 (a.a. 740-753) Peptide||50 μg||AB, E|
|phospho-DISC1 (Ser-710) Peptide||50 μg||AB, E|
|Anti-Rabbit Ig Light Chain Specific:HRP Mouse mAb||100 μl||WB, E, ICC, IHC||Rb|
The DISC1 phospho-regulation antibody kit can be used to detect phosphorylation of Ser-710 relative to total DISC1 expression. The kit includes two rabbit polyclonal antibodies to detect total expression of DISC1 and secondary reagents for detection of rabbit polyclonal antibody in western blot or ELISA. In addition, the kit includes peptides for blocking experiments or use as ELISA positive controls.