Catalog # DK6630

mDia Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution
ELISA, WB

Species Reactivity

Hu, Rt, Ms, Ck, F

$395


DATASHEET  

KIT SUMMARY

The mDia phospho-regulation antibody sampler kit can be used to detect the expression levels of mDia1, mDia2, and mDia3. In addition, the kit contains phospho-specific antibodies to detect phoshorylation of Thr-1061 on mDia2, and phosphorylation of Ser-196 in mDia3. The latter antibody detects a site that is well conserved in mDia1 (Ser-171) and mDia2 (Ser-202), so the antibody may be useful for detection of phosphorylation of these sites as well.

Western blot analysis of mDia2 expression in rat PC12 (lane 1), human A431 (lane 2), mouse brain (lane 3), and rabbit spleen fibroblasts (lane 4). The blots were probed with anti-mDia2 (C-terminal region) at 1:500.

Western blot analysis of mDia3 expression in human Jurkat cells treated with Calyculin A (100 nM) (lanes 1-4). The blots were treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal anti-mDia3 (C-terminus; DP4511) (lanes 1 & 2) and anti-phospho-mDia3 (Ser-196; DP4521) (lanes 3 & 4).



Rabbit polyclonal, affinity-purified antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

DP3491 Li X, et al. (2017) Haematologica. 102(6):984. ICC: fetal liver cells
DP3491 Monzo, P. et al. (2016) Mol Biol Cell. 27(8):1246. WB: rat C6 glioma, shRNA knockdown
DP3491 Spence, EF et al. (2016) J Neurosci.36(37):9696. WB: mouse postnatal brain
DP3491 Belin, BJ et al. (2015) Elife. Aug. 19;4:e07735. WB: human HeLa, shRNA knockdown
DP4511 Vitiello, E. et. al(2014) Nat Commun. 18(5):5826 WB: HeLa, HCE
DP3491 Sandbo, N. et al. (2013) J Biol Chem 288(22):15466. WB : human lung fibroblasts

This kit contains:

CATALOG #DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
mDia1 (a.a. 66-77) Rabbit pAb50 μlWB, EHu, Rt, Ms140
mDia2 (C-terminal region) Rabbit pAb50 μlWB, EHu, Rt, Ms135
mDia2 (Thr-1061), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck, F135
mDia3 (C-terminus) Rabbit pAb50 μlWB, EHu, Rt, Ms130
mDia3 (Ser-196)[conserved site], phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms130