Catalog # CK7680

γ-Catenin Phospho-Regulation Staining Kit

Immunocytochemistry Kit

Application / Dilution
ELISA, WB, IP, ICC, IHC

Species Reactivity

Hu, Rt, Ms, Rb

$355


DATASHEET  

KIT SUMMARY

The γ-Catenin phospho-regulation kit can be used for immunocytochemical co-localization of γ-Catenin phosphorylation at Tyr-550 compared to the total expression of γ-Catenin. The kit includes rabbit polyclonal and mouse monoclonal antibodies, along with goat-anti-rabbit conjugated to DyLight® 594 and goat anti-mouse conjugated to DyLight® 488 for dual labeling experiments.

Immunocytochemical labeling of phosphorylated γ-Catenin in control (Top) and pervanadate-treated (Bottom) A431 cells. The cells were labeled with mouse monoclonal γ-Catenin (CM1111) and rabbit polyclonal γ-Catenin (Tyr-550) antibodies, then the antibodies were detected using Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594. The overlay showing co-labeling between the two antibodies is shown to the right.



Mouse monoclonal, rabbit polyclonal, and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

CM1111 Cooksley-Decasper, S. et al. (2012) PLoS One 7(10):e47985. WB: human macrophages

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYICC DILUTION
γ-Catenin (C-terminal) Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms1:50
γ-Catenin (Tyr-550), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms 1:50
Anti-Mouse Ig:DyLight® 488 Goat pAb100 μlICC, IHCMs1:200
Anti-Rabbit Ig:DyLight® 594 Goat pAb100 μlICC, IHCRb1:200