Catalog # CK7680

γ-Catenin Phospho-Regulation Staining Kit

Immunocytochemistry Kit

DATASHEET  




$355
Plakoglobin (γ-Catenin) is a catenin family member identified as a component of desmosomes. γ-Catenin has high homology to β-catenin and, like β-catenin, it can associate with the cadherins, E-cadherin and N-cadherin. One molecule of α-catenin and at least one molecule of β-catenin and γ-Catenin simultaneously bind to a single cadherin molecule. A 19-amino acid sequence of desmoglein was found to be critical for binding of γ-Catenin. Similar catenin-binding domains found in cadherins suggest a common mechanism for γ-Catenin localization to both adherens junctions and desmosomes. Phosphorylation of tyrosine residues in γ-Catenin can modify its interactions with other proteins. Phosphorylation of tyrosine 644 decreases γ-Catenin association with α-catenin, but increases binding to desmoplakin. Fer kinase can phosphorylate tyrosine 550, which increases γ-Catenin binding to α-catenin. Thus, tyrosine phosphorylation may be important for regulation of γ-Catenin protein-protein interactions within desmosomal complexes.
References
McCrea, P.D. et al. (1991) Science 254:1359.
Miravet, S. et al. (2003) Mol. Cell. Biol. 23(20) :7391.

Immunocytochemical labeling of phosphorylated γ-Catenin in control (Top) and pervanadate-treated (Bottom) A431 cells. The cells were labeled with mouse monoclonal γ-Catenin (CM1111) and rabbit polyclonal γ-Catenin (Tyr-550) antibodies, then the antibodies were detected using Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594. The overlay showing co-labeling between the two antibodies is shown to the right.



Mouse monoclonal, rabbit polyclonal, and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

CM1111 Cooksley-Decasper, S. et al. (2012) PLoS One 7(10):e47985. WB: human macrophages

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYICC DILUTION
γ-Catenin (C-terminal) Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms1:50
γ-Catenin (Tyr-550), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms 1:50
Anti-Mouse Ig:DyLight® 488 Goat pAb100 μlICC, IHCMs1:200
Anti-Rabbit Ig:DyLight® 594 Goat pAb100 μlICC, IHCRb1:200
KIT SUMMARY

The γ-Catenin phospho-regulation kit can be used for immunocytochemical co-localization of γ-Catenin phosphorylation at Tyr-550 compared to the total expression of γ-Catenin. The kit includes rabbit polyclonal and mouse monoclonal antibodies, along with goat-anti-rabbit conjugated to DyLight® 594 and goat anti-mouse conjugated to DyLight® 488 for dual labeling experiments.