Catalog # CK7650

δ1-Catenin Phospho-Regulation Staining Kit

Immunocytochemistry Kit



Catenins have emerged as molecular sensors that integrate cell-cell junctions and cytoskeletal dynamics with signaling pathways that control morphogenesis and cell to cell communication. δ1-Catenin (p120 catenin) is a catenin family member which contains an N-terminal coiled-coil domain, a regulatory domain containing multiple phosphorylation sites, and a central Armadillo repeat domain. δ1-Catenin regulates E-cadherin turnover, and has both positive and negative effects on cadherin-mediated adhesion. Actin dynamics are also regulated by δ1-Catenin, which can modulate RhoA, Rac, and cdc42 activity. δ1-Catenin is phosphorylated at multiple tyrosine, serine, and threonine sites both in vitro and in vivo. High levels of δ1-Catenin phosphorylated at Tyr-228 are commonly seen in several carcinoma cell lines and after EGFR activation. Many other tyrosine sites are also phosphorylated in the N-terminal region, including Tyr-96, Tyr-112, Tyr-280, and Tyr-302. In addition, Thr-310 and Thr-916 are constituitively phosphorylated in many cell types, however this phosphorylation may occur only in δ1-Catenin associated with the plasma membrane.

Mariner, D.J. et al. (2001) J. Biol. Chem. 276:28006.
Reynolds, A.B. & Roczniak-Ferguson, A. (2004) Oncogene 23:7947.
Fukumoto, Y. et al. (2008) Exp. Cell Res. 314:52.

Immunocytochemical labeling of phosphorylated δ1-Catenin in A431 control (top) or treated with pervanadate (bottom). The fixed cells were labeled with mouse monoclonal antibodies to δ1-Catenin (a.a. 275-285), δ1-Catenin (Tyr-228), δ1-Catenin (Tyr-280), and δ1-Catenin (Tyr-302). The antibodies were detected using Goat anti-Mouse secondary antibodies conjugated to DyLight® 594.

Mouse monoclonal and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

δ1-Catenin (a.a. 275-285) Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms1:100
δ1-Catenin (Tyr-228), phospho-specific Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms1:100
δ1-Catenin (Tyr-280), phospho-specific Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms1:100
δ1-Catenin (Tyr-302), phospho-specific Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms1:100
Anti-Mouse Ig:DyLight® 488 Goat pAb100 μlICC, IHCMs1:200
Anti-Mouse Ig:DyLight® 594 Goat pAb100 μlICC, IHCMs1:200

The δ1-Catenin phospho-regulation kit can be used for immunocytochemical labeling of phosphorylation at Tyr-228, Tyr-280, and Tyr-302 in δ1-Catenin. The kit includes a monoclonal antibody to examine the pattern of expression of the total population of δ1-Catenin. In addition, secondary reagents conjugated to DyLight® 488 or DyLight® 594 are included for labeling primary antibodies for detection using green (493Ex/518Em) or red (593Ex/618Em) filter sets.