Catalog # CK7650

δ1-Catenin Phospho-Regulation Staining Kit

Immunocytochemistry Kit

Application / Dilution
ELISA, WB, ICC, IHC, IP

Species Reactivity

Hu, Rt, Ms

$455


DATASHEET  

KIT SUMMARY

The δ1-Catenin phospho-regulation kit can be used for immunocytochemical labeling of phosphorylation at Tyr-228, Tyr-280, and Tyr-302 in δ1-Catenin. The kit includes a monoclonal antibody to examine the pattern of expression of the total population of δ1-Catenin. In addition, secondary reagents conjugated to DyLight® 488 or DyLight® 594 are included for labeling primary antibodies for detection using green (493Ex/518Em) or red (593Ex/618Em) filter sets.

Immunocytochemical labeling of phosphorylated δ1-Catenin in A431 control (top) or treated with pervanadate (bottom). The fixed cells were labeled with mouse monoclonal antibodies to δ1-Catenin (a.a. 275-285), δ1-Catenin (Tyr-228), δ1-Catenin (Tyr-280), and δ1-Catenin (Tyr-302). The antibodies were detected using Goat anti-Mouse secondary antibodies conjugated to DyLight® 594.



Mouse monoclonal and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYICC DILUTION
δ1-Catenin (a.a. 275-285) Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms1:100
δ1-Catenin (Tyr-228), phospho-specific Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms1:100
δ1-Catenin (Tyr-280), phospho-specific Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms1:100
δ1-Catenin (Tyr-302), phospho-specific Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms1:100
Anti-Mouse Ig:DyLight® 488 Goat pAb100 μlICC, IHCMs1:200
Anti-Mouse Ig:DyLight® 594 Goat pAb100 μlICC, IHCMs1:200