Catalog # CK7610

β-Catenin Tyr-142 & Tyr-654 Phosphorylation Staining Kit

Immunocytochemistry Kit

Size Kit

DATASHEET  




$405

β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn Kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions, leading to changes in cell adhesion.

References
CP1081 Qi, F. et al. (2013) Am J Pathol. 183(5):1654. WB: human mesothelial
CP4021 Condello, S. et al. (2013) FASEB Journal 27(8): 3100. WB: ovarian cancer cells
CP1081 Krejci, P. et al. (2012) PLoS ONE. 7(4):e35826. WB: rat chondrosarcoma cells
CP1081 Beard, R.S. et al. (2011) Blood. 118(7):2007. ELISA: mouse microvascular ECs

Immunocytochemical labeling of phosphorylated β-Catenin in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts (top row) and A431 cells (bottom row). The fixed cells were labeled with mouse monoclonal antibody to β-Catenin (CM1811) (top and bottom left), and phospho-specific β-Catenin (Tyr-142) (top right) or β-Catenin (Tyr-654) (bottom right). The antibodies were detected using Goat anti-Mouse DyLight® 488 or Goat anti-Rabbit DyLight® 594.



Mouse monoclonal, rabbit polyclonal, and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

CP1081 Tsuneki, M. et al. (2014) Mol Cell Biol. 34(24): 4485. (IF: mouse EOMA, brain endothelial cells)
CP4021 Lu, Y. et al.(2014) Stem Cells Dev. 23(15):1755. (IF: chicken embryonic fibroblasts)
CM1181 Condello, S. et al. (2013) FASEB J 27(8):3100. (WB & IHC: Ovarian Cancer Cells)
CM1181 Yakubov, B. et al. (2013) Neoplasia 15(6):609. (WB: Human Ovarian Cancer Cells)
CP1081 Qi, F. et al. (2013) Am J Pathol. 183(5):1654. (WB: human mesothelial)
CP4021 Condello, S. et al. (2013) FASEB Journal 27(8): 3100. (WB: ovarian cancer cells)
CP1081 Krejci, P. et al. (2012) PLoS ONE. 7(4):e35826. (WB: rat chondrosarcoma cells)
CP1081 Beard, R.S. et al. (2011) Blood. 118(7):2007. (ELISA: mouse microvascular ECs)

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYICC DILUTION
β-Catenin Mouse mAb50 μlWB, E, IP, ICC, IHCHu, Rt, Ms1:250
β-Catenin (Tyr-142)/[γ-Catenin (Tyr-133)], phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms 1:100
β-Catenin (Tyr-654)/[γ-Catenin (Tyr-644)], phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck, Fr 1:200
Anti-Mouse Ig:DyLight® 488 Goat pAb100 μlICC, IHCMs1:200
Anti-Rabbit Ig:DyLight® 594 Goat pAb100 μlICC, IHCRb1:200
KIT SUMMARY

The β-Catenin phospho-regulation kit can be used for immunocytochemical co-localization of β-Catenin phosphorylation at Tyr-142 and Tyr-654 relative to total β-Catenin. The kit includes rabbit polyclonal and mouse monoclonal antibodies, along with goat-anti-rabbit conjugated to DyLight® 594 and goat anti-mouse conjugated to DyLight® 488 for dual labeling experiments.