Catalog # CK6940

Crk Phospho-Regulation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

The Crk family of adaptor proteins (Crk I, Crk II and CrkL) are Src Homology 2 (SH2) and Src Homology 3 (SH3) domain-containing proteins that form protein complexes important for transmiting signals downstream of tyrosine kinases. Both Crk II and CrkL are composed of a single SH2 domain, followed by two tandem SH3 domains. Crk II is also alternatively spliced to a minor product, Crk I, which is structurally and functionally more similar to the v-Crk oncogene. Both Crk II and CrkL are ubiquitously expressed and their SH domains are highly homologous, however both are required for mouse development and have distinct non-overlapping phenotypes in knockout mice. Phosphorylation may be important for regulating Crk activity. Crk II Tyr-221 (CrkL Tyr-207) phosphorylation is a negative regulatory site, while Crk Tyr-251 phosphorylation in the SH3 domain is a positive regulatory site. EGF stimulation induces phosphorylation of Tyr-251, which increases binding of Crk to the SH2 domain of Abl, and promotes transactivation of Abl.

References
Feller, S.M. (2001) Oncogene. 20(44):6348.
Feller, S.M. & Lewitzky, M. (2006) Curr. Pharm. Des. 12(5):529.
Sriram, G. et al. (2011) Oncogene 30(46):4645.

Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-6). The blot was treated with alkaline phosphatase to dephosphorylate Crk (lanes 2, 4 & 6), then the blot was probed with rabbit polyclonals CrkL (C-terminus) CP3081 (lanes 1 & 2), Crk (Tyr-251) phospho-specific CP3091 (lanes 3 & 4), and mouse monoclonal anti-Crk II (C-terminal region) (lanes 5 & 6).

Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate CrkL (lanes 2 & 4), then the blot was probed with rabbit polyclonals CrkL (C-terminus) CP3081 (lanes 1 & 2) and CrkL (Tyr-207) phospho-specific CP4671 (lanes 3 & 4).



Rabbit polyclonal and Mouse monoclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

CATALOG #DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Crk II (C-terminal region) Mouse mAb50 μlWB, E, IPHu, Rt, Ms40
Crk II (Tyr-221), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck, F40
Crk (Tyr-251), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck, F38/40
CrkL (C-terminus) Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck, F38
CrkL (Tyr-207), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck, F38
KIT SUMMARY

The Crk phospho-regulation antibody sampler kit can be used to detect phosphorylation of Tyr-221 and Tyr-251 in Crk II, as well as phosphorylation of Tyr-207 and Tyr-251 in CrkL. The kit includes a rabbit polyclonal anti-CrkL and mouse monoclonal anti-Crk II antibody to detect Crk expression levels.