Catalog # CK6360


Caspase Family

Antibody Sampler Kit

$395

The caspases are a group of cysteine enzymes which cleave proteins in response to intrinsic and extrinsic pathways that cause apoptotic cell death. The caspases can be grouped into two subgroups based on their roles in apoptosis. Initiator caspases (caspases 2, 8, 9, and 10) are activated through the apoptosis-signaling pathways and activate the effector caspases (caspases 3, 6, and 7) which carry out apoptosis. Caspase cascades are initiated through assembly of multiprotein complexes that trigger activation of the initiator caspases, which are then released and are able to activate the downstream effector caspases.

References
Adams, J.M. & Cory S. (2002) Curr Opin Cell Biol. 14(6):715.
Shi Y. (2002) Mol Cell. 9(3):459.
Salvesen, G.S. & Riedl SJ. (2008) Adv Exp Med Biol. 615:13
Mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

Western blot analysis of Caspase expression in human Jurkat cells. The blots were probed with mouse monoclonal anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2) or mouse monoclonal anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4).

Western blot analysis of Caspase expression in human Jurkat cells. The blots were probed with mouse monoclonal anti-Caspase-7 at 1:500 (lane 1) and 1:1000 (lane 2) or mouse monoclonal anti-Caspase-8 at 1:250 (lane 3) and 1:500 (lane 4).



This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Caspase-3 (N-terminal region)Mouse mAb50 μlWB, E, ICCHu32
Caspase-6 (C-terminal region) Mouse mAb50 μlWB, EHu34
Caspase-7 (N-terminal region) Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms35
Caspase-8 (C-terminal region) Mouse mAb50 μlWB, EHu55
Anti-Mouse Ig:HRP Donkey pAb100 μlWB, EMs
KIT SUMMARY

The Caspase family antibody sampler kit can be used to detect the full length and cleaved products of Caspase-3, Caspase-6, Caspase-7, and Caspase-8. The kit also includes secondary reagent for detection of these antibodies.