Catalog # CK6260

E-Cadherin Phospho-Regulation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 (Tyr-835 in E-cadherin) can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

References
Takeichi, M. (1988) Development 102:639.
Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463.
Xu, Y. & Carpenter, G. (1999) J. Cell. Bioch. 75:264.
Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261.

Western blot image of human A431 cells that were probed with rabbit polyclonal anti-E-Cadherin (a.a. 774-786) at 1:250 (lane 1), 1:1000 (lane 2), and 1:4000 (lane 3) or mouse monoclonal anti-E-cadherin (Cytoplasmic) at 1:250 (lane 4) and 1:1000 (lane 5).

Formalin fixed, citric acid treated parafin sections of embryonic Rat E16 intestines. Sections were probed with anti-E-Cadherin (CM1681) then anti-mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).



Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

CP1921 Gu, H. et al. (2014) FASEB Journal 28(10):4223. WB: human small airway epithelial cells
CP1921 SalaheldeenE. et al. (2014) J Histo Cytochem. 62(9):632. WB: seminiferous tubules
CP1951 Wang, M. et al. (2014) Cell Mol Neurobiol. 34(1):123 WB: MN9D neurons
CM1681 Milara, J. et al. (2013) Thorax. 68(5):410-20. IF: human pulmonary tissue
CM1681 Vittal, R. et al. (2013) AJP Lung Cell Mol Phys 304(6):401. WB: Rat & Human Epithelial Cells
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
E-Cadherin (Cytoplasmic)Mouse mAb50 μlWB, E, IP, ICC, IHCHu, Rt, Ms120
E-Cadherin (a.a. 774-786) Rabbit pAb50 μlWB, EHu, Rt, Ms120
N-Cadherin (a.a. 853-864)[E-Cadherin (a.a. 828-839)] Rabbit pAb 50 μlWB, EHu, Rt, Ms130
N-Cadherin (Y860)[E-Cadherin (Y835)], phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms130
Anti-Mouse Ig:HRP Donkey pAb 100 μlWB, EMs
Anti-Rabbit Ig Light Chain Specific:HRP Mouse mAb 100 μlWB, E, ICC, IHCRb
KIT SUMMARY

The E-cadherin phospho-regulation antibody sampler kit can be used to examine phosphorylation of E-cadherin at Tyr-835. The kit includes monoclonal and polyclonal antibodies to monitor the total level of expression for E-Cadherin and secondary reagents for detection of these antibodies.