Catalog # CK6150

γ-Catenin Phospho-Regulation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

Plakoglobin (γ-catenin) is a catenin family member identified as a component of desmosomes. γ-Catenin has high homology to β-catenin and, like β-catenin, it can associate with the cadherins, E-cadherin and N-cadherin. One molecule of α-catenin and at least one molecule of β-catenin and γ-catenin simultaneously bind to a single cadherin molecule. A 19-amino acid sequence of desmoglein was found to be critical for binding of γ-catenin. Similar catenin-binding domains found in cadherins, suggest a common mechanism for γ-catenin localization to both adherens junctions and desmosomes. Phosphorylation of tyrosine residues (Tyr-133, Tyr-480, Tyr-550, Tyr-644) in γ-catenin can modify its interactions with other proteins. Phosphorylation of Tyr-644 decreases γ-catenin association with α-catenin, but increases binding to desmoplakin. Fer kinase can phosphorylate Tyr-550, which increases γ-catenin binding to α-catenin. Thus, tyrosine phosphorylation may be important for regulation of γ-catenin protein-protein interactions within desmosomal complexes.

References
Miravet, S. et al. (2003) Mol. Cell. Biol. 23(20) :7391.
Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287.
Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225.
Rhee, J. et al. (2007) Nat. Cell Biol. 9(8):883.

Western blot analysis of anti-γ-Catenin (C-terminal) immunoprecipitates from pervanadate-treated A431. The immunoprecipitates were untreated (lanes 1,3,7) or treated with alkaline phosphatase (lanes 2,4,8). The blots were probed with γ-Catenin (a.a. 545-555), γ-Catenin (Tyr-550) or γ-Catenin (C-terminal) antibodies. The anti-γ-Catenin (Tyr-550) was used in the presence of γ-Catenin (Tyr-550) (lane 5) or γ-Catenin (Tyr-644) (lane 6) peptides.

Immunocytochemical labeling of phosphorylated γ-Catenin in control (Top) and pervanadate-treated (Bottom) A431 cells. The cells were co-labeled with mouse monoclonal γ-Catenin (CM1111) or rabbit polyclonal γ-Catenin (Tyr-550) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy2 or Cy3.



Rabbit polyclonal and mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

CP1081 Tsuneki, M. et al. (2014) Mol Cell Biol. 34(24): 4485 IF: mouse EOMA, brain endothelial cells
CP4021 Lu, Y. et al.(2014) Stem Cells Dev. 23(15):1755. IF: chicken embryonic fibroblasts
CP1081 Qi, F. et al. (2013) Am J Pathol. 183(5):1654. WB: human mesothelial
CP4021 Condello, S. et al. (2013) FASEB Journal 27(8): 3100. WB: ovarian cancer cells
CM1111 Cooksley-Decasper, S. et al. (2012) PLoS One 7(10):e47985. WB: human macrophages
CP1081 Krejci, P. et al. (2012) PLoS ONE. 7(4):e35826. WB: rat chondrosarcoma cells
CP2971 Cooksley-Decasper, S. et al. (2012) PLoS One 7(10):e47985. WB: human macrophages
CP1081 Beard, R.S. et al. (2011) Blood. 118(7):2007. ELISA: mouse microvascular ECs
CP2961 Beard, R.S. et al. (2011) Blood. 118(7):2007. ELISA: mouse microvascular ECs
CP2961 Funakoshi S et al (2010) Am J Phys Gast Liv Phys 299(5):1054 WB: human colon cancer cells

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
β-Catenin (Tyr-142)/[γ-Catenin (Tyr-133)], phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms92
β-Catenin (Tyr-489)/[γ-Catenin (Tyr-480)], phospho-specific Rabbit pAb 50 μlWB, E, ICCHu, Rt, Ms92
β-Catenin (Tyr-654)/[γ-Catenin (Tyr-644)], phospho-specific Rabbit pAb 50 μlWB, E, ICCHu, Rt, Ms, Ck, Fr92
γ-Catenin (C-terminal) Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms84
γ-Catenin (a.a. 545-555) Rabbit pAb50 μlWB, EHu, Rt, Ms84
γ-Catenin (Tyr-550), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms84
KIT SUMMARY

The γ-Catenin phospho-regulation antibody sampler kit can be used to examine phosphorylation of γ-Catenin at Tyr-133, Tyr-480, Tyr-550 and Tyr-644. The kit also includes monoclonal and polyclonal antibodies to monitor total expression levels of γ-Catenin.