Plakoglobin (γ-catenin) is a catenin family member identified as a component of desmosomes. γ-Catenin has high homology to β-catenin and, like β-catenin, it can associate with the cadherins, E-cadherin and N-cadherin. One molecule of α-catenin and at least one molecule of β-catenin and γ-catenin simultaneously bind to a single cadherin molecule. A 19-amino acid sequence of desmoglein was found to be critical for binding of γ-catenin. Similar catenin-binding domains found in cadherins, suggest a common mechanism for γ-catenin localization to both adherens junctions and desmosomes. Phosphorylation of tyrosine residues (Tyr-133, Tyr-480, Tyr-550, Tyr-644) in γ-catenin can modify its interactions with other proteins. Phosphorylation of Tyr-644 decreases γ-catenin association with α-catenin, but increases binding to desmoplakin. Fer kinase can phosphorylate Tyr-550, which increases γ-catenin binding to α-catenin. Thus, tyrosine phosphorylation may be important for regulation of γ-catenin protein-protein interactions within desmosomal complexes.
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Rhee, J. et al. (2007) Nat. Cell Biol. 9(8):883.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:CP1081 Tsuneki, M. et al. (2014) Mol Cell Biol. 34(24): 4485 IF: mouse EOMA, brain endothelial cells
CP4021 Lu, Y. et al.(2014) Stem Cells Dev. 23(15):1755. IF: chicken embryonic fibroblasts
CP1081 Qi, F. et al. (2013) Am J Pathol. 183(5):1654. WB: human mesothelial
CP4021 Condello, S. et al. (2013) FASEB Journal 27(8): 3100. WB: ovarian cancer cells
CM1111 Cooksley-Decasper, S. et al. (2012) PLoS One 7(10):e47985. WB: human macrophages
CP1081 Krejci, P. et al. (2012) PLoS ONE. 7(4):e35826. WB: rat chondrosarcoma cells
CP2971 Cooksley-Decasper, S. et al. (2012) PLoS One 7(10):e47985. WB: human macrophages
CP1081 Beard, R.S. et al. (2011) Blood. 118(7):2007. ELISA: mouse microvascular ECs
CP2961 Beard, R.S. et al. (2011) Blood. 118(7):2007. ELISA: mouse microvascular ECs
CP2961 Funakoshi S et al (2010) Am J Phys Gast Liv Phys 299(5):1054 WB: human colon cancer cells
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||MW (kDa)|
|β-Catenin (Tyr-142)/[γ-Catenin (Tyr-133)], phospho-specific Rabbit pAb||50 μl||WB, E, ICC||Hu, Rt, Ms||92|
|β-Catenin (Tyr-489)/[γ-Catenin (Tyr-480)], phospho-specific Rabbit pAb||50 μl||WB, E, ICC||Hu, Rt, Ms||92|
|β-Catenin (Tyr-654)/[γ-Catenin (Tyr-644)], phospho-specific Rabbit pAb||50 μl||WB, E, ICC||Hu, Rt, Ms, Ck, Fr||92|
|γ-Catenin (C-terminal) Mouse mAb||50 μl||WB, E, IP, ICC||Hu, Rt, Ms||84|
|γ-Catenin (a.a. 545-555) Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms||84|
|γ-Catenin (Tyr-550), phospho-specific Rabbit pAb||50 μl||WB, E, ICC||Hu, Rt, Ms||84|
The γ-Catenin phospho-regulation antibody sampler kit can be used to examine phosphorylation of γ-Catenin at Tyr-133, Tyr-480, Tyr-550 and Tyr-644. The kit also includes monoclonal and polyclonal antibodies to monitor total expression levels of γ-Catenin.