Catalog # AK6970

Argonaute 2 Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution
WB, E, ICC, IHC

Species Reactivity

Hu, Rt, Ms, Ck, F, Rb, Bv

$395


DATASHEET  

KIT SUMMARY

The argonaute 2 (Ago2) phospho-regulation antibody sampler kit can be used to detect phosphorylation of Ser-389 and Tyr-393 relative to total Ago2 expression levels. The kit includes rabbit polyclonal phospho-specific antibodies to Ser-389 and Tyr-393, as well as rat monoclonal and rabbit polyclonal antibodies for detection of total Ago2. The kit also includes anti-Rabbit Light Chain specific:HRP and anti-Rat Ig:HRP secondary reagents for detection of antibodies in western blot, ELISA, or immunocytochemistry.

Western blot analysis of mouse recombinant Ago2 full length protein (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonal anti-Ago2 (AP5281) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Ser-387) phospho-specific antibody (lanes 3 & 4).

Western blot analysis of human A431 cells treated with EGF (100 ng/ml for 60 min.) (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rat monoclonal anti-Ago2 (AM5271) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Tyr-393) phospho-specific antibody (lanes 3 & 4).



Rat monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Argonaute 2 (N-terminus) Rat mAb50 μlWB, EHu, Bv97
Argonaute 2 (a.a. 389-398) Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck F97
Argonaute 2 (Ser-387), phospho-specific Rabbit pAb 50 μlWB, EHu, Rt, Ms, Ck, F97
Argonaute 2 (Tyr-393), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck, F97
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb 100 μlWB, E, ICC, IHCRb
Anti-Rat IgG Light Chain:HRP Goat pAb100 μlWB, E, ICC, IHCRt