Catalog # AK6670

Arp2/3 Complex Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. Arp2 has two phosphosites, Thr-237 and Thr-238, that are evolutionarily conserved, and are phosphorylated along with Tyr-202 in response to growth factor stimulation. p41-Arc (Arpc1b) subunit Arpc1 has two isoforms in humans, Arpc1a and Arpc1b. PAK1 can bind and phosphorylate Thr-21 in Arpc1b leading to growth factor-stimulated cell motility. In addition, Arpc1b colocalizes with γ-tubulin at centrosomes and stimulates Aurora A activity.

References
Kelleher, J.F. et al. (1995). J Cell Biol. 131(2):385.
Vadlamudi, R.K. et al. (2004) EMBO Rep. 5(2):154.
LeClaire, L.L. et al (2008). J Cell Biol. 182(4):647.
Molli, P.R. et al. (2010) J Cell Biol. 190(1):101.

Western blot of Arpc1b phosphorylation in A431 stimulated with calyculin A (100 nM) for 30 min (lanes 1 & 3). The blots were treated with lambda phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Arpc1b (C-terminal region) (lanes 1 & 2) or anti-Arpc1b (Thr-21) (lanes 3 & 4).

Immunocytochemical labeling of Arp2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with Arp2 (C-terminal region) and Arp2 (Thr-237/Thr-238) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to Cy3.



Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

AP3871 Osma-Garcia, I.C. et al. (2015) Eur J Immunol. doi: 10.1002 WB: mouse macrophages
AP4351 Kim, Y.B. et al. (2015) J Biol Chem. 290(34):21076 WB: PC3 cells
AP3861 Osma-Garcia, I.C. et al. (2015) Eur J Immunol. doi: 10.1002 WB: mouse macrophages
AP4321 Ho, H. et al. (2013) Pigment Cell Melanoma Res 26(2):218. WB: human A375 melanoma
AP4351 Ho, H. et al. (2013) Pigment Cell Melanoma Res 26(2):218. WB: human A375 melanoma
AP3861 Spillane, M. et al. (2012) J Neurosci. 32(49):17671. ICC: Chicken embryo neuron
AP3871 Kalwa, H. & Michel, T. (2011) J Biol Chem. 286(3):2320. WB: bovine aortic endothelial cells
AP3861 Kalwa, H. & Michel, T. (2011) J Biol Chem. 286(3):2320. WB: bovine aortic endothelial cells
AP3861 Spillane, M. et al. (2011) Dev Neurobiol. 71(9):747. ICC: chick dorsal root ganglia

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Arp2 (C-terminal region) Rabbit pAb50 μlWB, E, IP, ICCHu, Rt, Ms, Ck, Fr44
Arp2 (Thr-237/Thr-238), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck44
Arp3 (C-terminal region) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck, F47
Arpc1b/p41-Arc (C-terminal region) Rabbit pAb50 μlWB, E, IP, ICCHu, Rt, Ms, Ck41
Arpc1b/p41-Arc (Thr-21), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck, F41
KIT SUMMARY

The Arp2/3 complex kit can be used to detect expression levels of Arp2, Arp3, and Arpc1b in many different species. In addition, phospho-specific antibodies are included to detect phosphorylation of Arp2 (Thr-237/Thr-238) and Arpc1b (Thr-21).