Catalog # AK6480

α-Actinin 4 Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution

Species Reactivity

Hu, Rt, Ms, Rb




The α-Actinin 4 phospho-regulation antibody sampler kit can be used to examine phosphorylation of α-Actinin 4 at Tyr-4. The kit includes polyclonal anti-α-Actinin 4 antibody to monitor total expression levels of α-Actinins, peptides for blocking experiments, and HRP-conjugated secondary reagent for primary antibody detection.

Western blot analysis of α-actinin 4 in A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 2, 5, 6) or after immunoprecipitation using α-actinin (C-terminal region) antibody in the absence (lanes 3 & 7) or presence of pervanadate-treated A431 cell lysate (lanes 4 & 8). Some lanes of the blot were treated with alkaline phosphatase (lanes 2 & 6). The blots were probed with anti-α-actinin (C-terminal region) or anti-α-actinin 4 (Tyr-4), phospho-specific.

Western blot analysis of α-actinin in human A431 cells (lanes 1 & 3) and rabbit spleen fibroblasts (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-α-actinin 4 (a.a. 2-11) or mouse monoclonal anti-α-actinin (C-terminal region).

Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagent is supplied in the same buffer without azide, and the peptides are supplied in PBS and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

AP4241 Kojima, S. et al. (2014) Am J Nephrol. 39(1):36-45 WB: human kidney cells
AP4271 Kojima, S. et al. (2014) Am J Nephrol. 39(1):36-45 WB: human kidney cells
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

α-Actinin 4 (a.a. 2-11) Rabbit pAb50 μlWB, EHu, Rt, Ms104
α-Actinin 4 (a.a. 2-11) Peptide50 μgAB, E
α-Actinin 4 (Tyr-4), phospho-specific Rabbit pAb 50 μlWB, EHu, Rt, Ms104
phospho-α-Actinin 4 (Tyr-4) Peptide50 μgAB, E
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb100 μlWB, E, ICC, IHCRb