Catalog # FK7231

Fibronectin (Human) Paired ELISA KIT

ELISA Kit

Application / Dilution
ELISA

Species Reactivity

Human

$395


DATASHEET  

 

KIT SUMMARY

The Fibronectin ELISA kit can be used for capture of human Fibronectin in ELISA sandwich assays of cell lysates, serum, and other liquid biological samples. The kit includes mouse monoclonal anti-Fibronectin (FM013P) capture antibody and biotin conjugated anti-Fibronectin (FM022B) dectection antibody along with a Stretavidin:HRP reagent (SS300K) for biotin detection.

Representative Standard Curve using mouse monoclonal anti-fibronectin (FM013P) for ELISA capture of human recombinant fibronectin protein. Capture was detected by using anti-fibronectin (FM022B) biotin conjugate followed by streptavidin conjugated to HRP (SS300K).



Mouse monoclonal capture antibody is supplied in phosphate-buffered saline and 0.05% sodium azide. Mouse monoclonal detection antibody is supplied in phosphate-buffered saline, 50% glycerol, and 0.05% sodium azide. Streptavidin:HRP is supplied in phosphate-buffered saline, 50% glycerol, and 0.01% Thimerosal. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYDILUTION
Fibronectin (Cell/Heparin2 Binding region) Mouse mAb50 μlELISA (Cap)Hu1:250
Fibronectin:Biotin Mouse mAb50 μlELISA (Det)Hu1:1000
SS300K
Streptavidin:HRP50 μlBiotin (Det)1:2000