Catalog # CM1681

E-Cadherin (Cytoplasmic) Antibody

Mouse Monoclonal

Application / Dilution

ELISA	1:2000
ICC	1:250
IHC	1:50
IP	1:100
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 120 kDa

Isotype IgG1

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

References

Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261.
Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463.
Takeichi, M. (1988) Development 102:639.

Western blot image of human A431 cells treated with pervanadate (1 mM) for 30 min (lanes 1 & 3) then treated with akaline phosphatase (lanes 2 & 4). Blots were probed with anti-E-Cadherin (Cytoplasmic) and anti-N-Cadherin (Tyr-860)/E-Cadherin (Tyr-835) conserved site.

Formalin fixed, citric acid treated parafin sections of embryonic Rat E16 intestines. Sections were probed with anti-E-Cadherin (CM1681) then anti-mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).

Clone (M168) was generated from a mouse recombinant E-Cadherin protein containing amino acids in the C-terminal region. This sequence is highly conserved in human and rat E-cadherin.

*For more information, see UniProt Accession P09803

Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This E-cadherin antibody detects a 120 kDa* protein in human A431 cells, and does not cross-react with VE-cadherin or N-cadherin.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Pastor-Cleriguesab, A et al. (2016) Curr Eye Res. 41(7):890. (IHC: bovine cornea)
Signorelli, P. et al. (2015) Nutr Cancer. 67(3):494. (WB: human HCT116 cells)
Liu, D. et al. (2015) Am J Pathol. 185(1):110. (ICC/IHC: rat Liver epithelial cells)
Milara, J. et al. (2015) COPD. 12(3):320. (ICC: human bronchial epithelial cells)
Milara, J. et al. (2014) Pulm Pharmacol Ther. 28(2):138. (Protein Array: human bronchial endothelial cells)
Milara, J. et al. (2013) Thorax. 68(5):410-20. (IF: human pulmonary tissue)
Vittal, R. et al. (2013) AJP Lung Cell Mol Phys 304(6):401. (WB: rat & human Epithelial Cells)

This kit contains: