Catalog # CP1191

β-Catenin (Tyr-86), phospho-specific Antibody

Rabbit Polyclonal

Application / Dilution
ELISA1:2000
ICC1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 92 kDa

$329


DATASHEET  

β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn Kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions, leading to changes in cell adhesion.

 

References
Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225.
Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287.
Roura, S. et al. (1999) J Biol Chem. 274(51) :36734.

Western blot of β-Catenin immunoprecipitated from A431 cells treated with pervanadate. The blots were probed with β-Catenin (Tyr-86) or β-Catenin (a.a. 649-661) antibodies. The immunoprecipitates were untreated (lanes 1 & 6) or treated with alkaline phosphatase (lanes 5 & 10), and the antibodies were incubated with β-Catenin (Tyr-86) (lanes 2,7) or β-Catenin (a.a. 649-661) (lanes 3,8) peptides or phospho-Tyr (lanes 4,9).

Immunocytochemical labeling of β-Catenin in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal β-Catenin (CM1181) or rabbit polyclonal β-Catenin (Tyr-86) or β-Catenin (Y654) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.



Phospho-β-Catenin (Tyr-86) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 86 of human β-Catenin. This peptide sequence is highly conserved in rat and mouse β-Catenin.

*For more information, see UniProt Accession P35222
Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose before affinity purification using phospho- β-Catenin (Tyr-86) peptide (without carrier). The antibody detects a 92kDa* protein corresponding to the molecular mass of β-Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells treated with pervanadate (1 mM for 30 min), but not in control cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: