Catalog # CM3561

δ1-Catenin (Tyr-228), phospho-specific Antibody

Mouse Monoclonal

Application / Dilution
ELISA1:2000
ICC1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 110 kDa

Isotype IgG1

$360


DATASHEET  

Catenins have emerged as molecular sensors that integrate cell-cell junctions and cytoskeletal dynamics with signaling pathways that control morphogenesis and cell to cell communication. δ1-Catenin (p120 catenin) is a catenin family member which contains an N-terminal coiled-coil domain, a regulatory domain containing multiple phosphorylation sites, and a central Armadillo repeat domain. δ1-Catenin regulates E-cadherin turnover, and has both positive and negative effects on cadherin-mediated adhesion. Actin dynamics are also regulated by δ1-Catenin, which can modulate RhoA, Rac and cdc42 activity. δ1-Catenin is phosphorylated at multiple tyrosine, serine and threonine sites both in vitro and in vivo. High levels of δ1-Catenin phosphorylated at Tyr-228 are commonly seen in several carcinoma cell lines and after EGFR activation. Many other tyrosine sites are also phosphorylated in the N-terminal region including Tyr-96, Tyr-112, Tyr-280, and Tyr-302. In addition, Thr-310 and Thr-916 are constituitively phosphorylated in many cell types, however this phosphorylation may occur only in δ1-Catenin associated with the plasma membrane.

 

References

Fukumoto, Y. et al. (2008) Exp. Cell Res. 314:52.
Reynolds, A.B. & Roczniak-Ferguson, A. (2004) Oncogene 23:7947.
Mariner, D.J. et al. (2001) J. Biol. Chem. 276:28006.

Western blot analysis of δ1-Catenin phosphorylation in A431 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1,3,5,7,9). The blot was then treated with alkaline phosphatase (lanes 2,4,6,8,10). Blots were probed with anti-δ1-Catenin (a.a. 275-285) (lanes 1 & 2), anti-δ1-Catenin (Tyr-96) (lanes 3 & 4), anti-δ1-Catenin (Tyr-228) (lanes 5 & 6), anti-δ1-Catenin (Tyr-280) (lanes 7 & 8) or anti-δ1-Catenin (Tyr-302) (lanes 9 & 10).

Immunocytochemical labeling of δ1-Catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal δ1-Catenin (a.a. 275-285), δ1-Catenin (Tyr-228), δ1-Catenin (Tyr-280), or δ1-Catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.



Clone M356 was generated from a phospho-δ1-Catenin (Tyr-228) synthetic peptide containing amino acid residues around tyrosine 228 of human δ1-Catenin. This peptide sequence is highly conserved in rat and mouse δ1-Catenin.

*For more information, see UniProt Accession O60716
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 110 kDa* protein corresponding to the molecular mass of δ1-Catenin on SDS-PAGE immunoblots of human A431 and HUVEC cells treated with pervanadate. This antibody does not detect this band after alkaline phosphatase treatment.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: