The CD44 ELISA kit can be used for capture of human CD44 protein in ELISA sandwich assays of cell lysates, serum, and other liquid biological samples. The kit includes mouse monoclonal anti-CD44 (CM591P) capture antibody and anti-CD44 (CM010D) Isotype IgG2b detection antibody along with anti-Mouse IgG2b specific:HRP (MS3231) secondary reagent.
Mouse monoclonal capture antibody is supplied in phosphate-buffered saline and 0.05% sodium azide. Mouse monoclonal detection antibody is supplied in phosphate-buffered saline, 50% glycerol, and 0.05% sodium azide. Anti-Mouse IgG2b:HRP is supplied in phosphate-buffered saline, 50% glycerol, and 1 mg/ml BSA. Store all at –20°C. Stable for 1 year
Comparison of quantitative western blot (A) and sandwich ELISA (C) measurement of CD44 protein expression in A549, SK-MES-1, H1299, and H1915 cell lines. The western blot (B) was probed with anti-CD44 mouse monoclonal (CM5911), and the ELISA used mouse monoclonal anti-CD44 (CM591P) for capture of CD44 native protein. Captured CD44 was detected by using mouse monoclonal IgG2b anti-CD44 (CM010D) followed by anti-Mouse IgG2b specific:HRP (MS3231).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot
mobilities of known proteins with similar MW.
This kit contains:
|CD44 (Hyaluron Binding Region) Mouse mAb||50 μl||ELISA (Cap)||Hu||1:250|
|CD44 (Extracellular region) Mouse mAb||50 μl||ELISA (Det)||Hu||1:2000|
|Anti-Mouse IgG2b specific:HRP Goat pAb||100 μl||Secondary||Ms||1:2000|