Catalog # PM1071

Paxillin Antibody

Mouse Monoclonal

Application / Dilution

ELISA	1:2000
ICC	1:100
IP	1:100
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck

MW 68 kDa

Isotype IgG1

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration.

References

Huang, C. et al. (2004) Cell Cycle 3(1):4-6.
Huang, C. et al. (2003) Nature 424:219-223.
Woodrow, M.A. (2003) Exp. Cell. Res. 287(2):325-338.

Western blot analysis of A431 cells (20 µg/lane) serum starved overnight and treated with EGF (100 ng/ml) for 5 min. The blot was probed with anti-Paxillin mouse monoclonal (PM1071) or anti-Paxillin (Ser-178) rabbit polyclonal (PP1051).

Immunocytochemical labeling of Ser-83 phosphorylated paxillin in rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal Paxillin (left) and rabbit polyclonal Paxillin (Ser-83, right) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3.

Clone (M107) was generated from full-length recombinant human paxillin.

*For more information, see UniProt Accession P49023

Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 68kDa* protein corresponding to the molecular mass of paxillin on SDS-PAGE immunoblots of A431 cells. Similar results were seen in various cells and tissues from rat, mouse, and chicken origin.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Stone, JD et al. (2016) J Non Invasive Vasc Invest. 1:2-9. (FC: rat ATr5 smooth muscle cells)
Dasgupta, SK et al. (2017) Cancer Med. 6(4):809. (WB: HMEC-1 cells)
Omori, K. et al. (2015) Bioch Biophys Res Com 458(4): 934. (IF: mouse NIH3T3 fibroblasts)
Cheng, I. et al. (2014) J Biol Chem. 289(39):26989 (IF: B16-F1 cells)
Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. (WB: rat C6 and human U373 gliomas)
Liu, H. et al. (2013) BMC Cancer. 13:80. (WB: human ovarian cancer cells)
Richard, D. et al. (2008) Acta Neurophathologica 115(2):205. (Immunocytochemistry)

This kit contains: