Catalog # GM3421

GM130 (C-terminal region) Antibody

Mouse Monoclonal

Application / Dilution

ELISA	1:1000
ICC	1:50
WB	1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 130 kDa

Isotype IgG1

Golgi Matrix Protein (GM130) is a peripheral cytoplasmic protein that is bound to Golgi membranes. It maintains cis-Golgi structure, and it regulates the disassembly and reassembly of the Golgi complex during mitosis. GM130 may also be important during docking and fusion of coatomer (COPI) coated vesicles to the Golgi membrane. The carboxy-terminal domain of GM130 is highly homologous to the human auto-antigen, golgin-95. GM130 interacts in a GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes. It has also been implicated in the activation of Ste-kinase, YSK1, during the golgi reorganization that occurs along with cell migration.

References

Weide, T. et al. (2001) EMBO Rep 2:336.
Nakamura, N. et al. (1995) J Cell Biol 131:1715.
Warren, G. (1993) Annual Rev Biochem 62:323.

Immunocytochemical labeling of GM130 in C2C12 cells. The cells were double-labeled with GM130 (golgi protein) mouse monoclonal and nSMase-3 rabbit polyclonal antibodies, then detected using appropriate secondary antibody conjugated to Cy2 or Cy3.

Western blot analysis of GM130 expression in human cells: MCF7 breast carcinoma (lane 1), A549 adenocarcinoma (lane 2), and MDA-MB-231 breast carcinoma (lane 3). The blot was probed with mouse monoclonal anti-GM130 (C-terminal region) at 1:500.

Clone M342 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human GM130. This sequence has high homology to similar regions in rat and mouse GM130.

*For more information, see UniProt Accession Q08379

Mouse monoclonal purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 130 kDa* protein corresponding to the apparent molecular mass of GM130 on SDS-PAGE immunoblots of mouse C2C12 cells. In immunocytochemistry, anti-GM130 specifically stains Golgi in mouse C2C12 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Lin, Q. et al. (2017) J Cell Sci. 130(22):3839. (ICC: human A549 cells)
Sun, A. et al. (2017) Autophagy. 13(3):522. (ICC: human A549 cells)
Vazquez-Calvo, A. et al. (2016) Front Microbiol. 7:612. (WB: bovine kideny cells)
Martín-Acebes, M.A. et al. (2014) J virology 88(20): 12041. (ICC: human HeLa cells)
Mart?n-Acebes, M.A. et al. (2011) PLoS ONE 6(9): e24970. (ICC: monkey Vero cell)

This kit contains: