Catalog # CM0361

CD147/Emmprin/Basigin (Extracellular region) M036 Antibody

Mouse Monoclonal

Application / Dilution

WB	1:2000
ELISA (Cap)	1:100
ICC	1:100
IP	1:100

Size 100 μl

Species Reactivity Hu

MW 38-58 kDa

Isotype IgG2a

CD147 (Basigin/EMMPRIN) is a type I integral membrane receptor protein belonging to the immunoglobulin superfamily. CD147 is a glycosylated protein with four known isoforms with isoform 2 being the most abundantly expressed. In many cancers, CD147 is overexpressed and stimulates the secretion of extracellular matrix metalloproteinases by tumor fibroblasts, which can promote tumor progression. CD147 is co-expressed with the amino acid transporter, CD98h, in metabolically active cells such as lymphocytes, macrophages, and tumor cells. CD147 interaction with caveolin and cyclophilins regulates its functions, and this protein may function outside of tumor cells through cell shedding and microvesicle release.

References

Miyauchi T. et al. (1990) J Biochem. 107(2):316.
Biswas, C. et al. (1995) Cancer Res. 55:434.
Xiong L. et al. (2014) Int J Mol Sci. 15(10):17411.

Western blot of lysates from BEAS normal lung cells (lane 1), NCIH28 mesothelial cells (lane 2), NCI-H1299 lung cancer cells (lane 3), LNCaP prostate cancer cells (lane 4), MCF7 breast cancer cells (lane 5), and A431 skin adenocarcinoma (lane 6). The blot was probed with mouse monoclonal anti-CD147 (CM0361) at 1:1000.

Immunocytochemical labeling of CD147 in paraformaldehyde fixed human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD147 (CM0361). The antibody was detected using goat anti-mouse Ig:DyLight® 594.

Representative Standard Curve using mouse monoclonal CD147 (CM0361) for ELISA capture of human recombinant CD147 extracellular region with a His tag. Capture was detected using anti-His tag antibody followed by appropriate secondary antibody HRP conjugate.

Clone (M036) was generated from a proprietary antigen related to native human CD147 expressed in NCI-H1915 lung cancer cell line.

*For more information, see UniProt Accession P35613

Mouse monoclonal, protein G purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M036 mouse monoclonal antibody detects a 38-58 kDa* protein on SDS-PAGE immunoblots of human BEAS, NCIH28, NCI-H1299, LNCaP, and A431 cells, as well as human brain tissues. The antibody works for western blot, immunoprecipitation, ELISA capture, and immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: