Catalog # CM5911

CD44 (Hyaluron Binding Region) M591 Antibody

Mouse Monoclonal

Application / Dilution
ELISA (Cap)1:100

Size 100 μl

Species Reactivity Hu

MW 80 kDa

Isotype IgG1



Cell surface adhesion protein CD44 is a ubiquitously expressed type I transmembrane protein that has important functions related to cell-cell adhesion and extracellular matrix interactions. The transmembrane protein is post-translationally modified at multiple sites by glycosylation and phosphorylation. CD44 ligands include hyaluronic acid, collagens, laminins, osteopontin, serglycin, and fibronectin. CD44 has been implicated in inflammatory cell functions as well as in tumor growth and metastasis. CD44 is overexpressed in many types of cancer; the interaction between CD44 and HER2 has been linked to an increase in ovarian carcinoma cell growth. CD44 interacts with ezrin, radixin, and moesin to link the actin cytoskeleton to the plasma membrane and the extracellular matrix. These interactions are critical for CD44 function in cell-cell adhesion and cell motility.


Cichy, J., et al. (2003) J Cell Biol. 161(5):839
Goodinson, S., et al. (1999) Mol Path. 52(4):189
Tsukita, S., et al. (1994) J Cell Biol. 126(2):391

Western blot analysis of denatured (lanes 1 & 3) and native (lanes 2 & 4) human MDA-MB-231 whole cell lysates. The blots were probed with mouse monoclonal anti-CD44 (CM5881) at 1:1000 (lanes 1 & 2) or mouse monoclonal anti-CD44 (CM5911) at 1:1000 (lanes 3 & 4).

Immunocytochemical labeling of CD44 in paraformaldehyde fixed human MCF7 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M591). The antibody was detected using goat anti-mouse DyLight® 594.

Clone M591 was generated from a proprietary antigen related to the hyaluron binding region of human CD44 from the MDA-MB-231 breast cancer cell line.

*For more information, see UniProt Accession P16070
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M591 detects 80-130 kDa* bands corresponding to the molecular mass of CD44 on SDS-PAGE immunoblots of denatured and native MDA-MB-231 cell lysates. The antibody also detects the denatured and native form of a recombinant human CD44 protein that includes only the hyaluron binding region. Clone M591 can be used in multiple applications including western blot, immunocytochemical labeling, ELISA, and immunoprecipitation, as well as for detecting CD44 in live, unfixed cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: