Catalog # VP2941

VEGFR-3 (a.a.1285-1298) Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt

MW 170 kDa

Vascular endothelial growth factor receptor-2 (VEGFR-2/Flk-1/KDR) is the primary receptor for VEGF in endothelial cells. Other VEGFR family members, VEGFR-1 (Flt-1) and VEGFR-3 (Flt-4), can also transduce the intracellular signals of VEGF. However, the role of VEGFR-1 is observed mainly during embryonic angiogenesis and VEGFR-3 signaling may be restricted to specific types of endothelial cells. Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr-951/996) and in the tyrosine kinase catalytic domain (Tyr-1054/1059). Other sites, Tyr-1175 and Tyr-1212 provide docking sites for downstream signaling molecules. Activation of VEGFR-2 also phosphorylates Tyr-801, leading to PI3-kinase-Akt activation and increases in endothelial nitric oxide synthase activity. Phosphorylation of mutliple sites in VEGFR-2 is required for downstream activation of several signaling pathways that control proliferation, chemotaxis, and sprouting during angiogenesis.

References

Garcia Blanes, M. et al. (2007) J Biol. Chem. 282(14):10660.
Kiselyov, A. et al. (2007) Expert Opin. Investig. Drugs 16(1):83.
Robinson, C.J. & Stringer, S.E. (2001) J. Cell Sci.114:853.
Meyer, M. et al. (1999) EMBO J. 18:363.
Dougher-Vermazen, M. et al. (1994) Bioch Biophys Res Com. 205:728.

Left: Western blot image of human K-562 cells (lanes 1 & 2) and HUVEC (lanes 3 & 4). The blots were probed with rabbit polyclonal anti-VEGFR-3 (a.a. 1285-1298) in the absence (lanes 1 & 3) or presence of blocking peptide (VX2945) (lanes 2 & 4). Right: Western blot image of GST-recombinant human VEGFR-1 (89 kDa), VEGFR-2 (110 kDa), and VEGFR-3 (86 kDa) C-terminal regions. The blot was probed with anti-VEGFR-3 (a.a. 1285-1298).

A synthetic peptide (coupled to carrier protein) corresponding to amino acids 1285 to 1298 in human VEGFR-3. This sequence has two amino acid differences from rat VEGFR-3, and five amino acid differences from mouse VEGFR-3.

*For more information, see UniProt Accession P35916

Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified with VEGFR-3 (a.a. 1285-1298) peptide. The purified antibody detects a 170 kDa* band corresponding to VEGFR-3 in western blots of human endothelial and K-562 cells, and shows strong reactivity to recombinant human VEGFR-3, but not VEGFR-1 or VEGFR-2.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: