Catalog # VP2921

VEGFR-2 (Tyr-801)[conserved site], phospho-specific Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
ICC	1:100
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 180 kDa

Vascular endothelial growth factor receptor-2 (VEGFR-2/Flk-1/KDR) is the primary receptor for VEGF in endothelial cells. Other VEGFR family members, VEGFR-1 (Flt-1) and VEGFR-3 (Flt-4), can also transduce the intracellular signals of VEGF. However, the role of VEGFR-1 is observed mainly during embryonic angiogenesis and VEGFR-3 signaling may be restricted to specific types of endothelial cells. Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr-951/996) and in the tyrosine kinase catalytic domain (Tyr-1054/1059). Other sites, Tyr-1175 and Tyr-1212 provide docking sites for downstream signaling molecules. Activation of VEGFR-2 also phosphorylates Tyr-801, leading to PI3-kinase-Akt activation and increases in endothelial nitric oxide synthase activity. Phosphorylation of mutliple sites in VEGFR-2 is required for downstream activation of several signaling pathways that control proliferation, chemotaxis, and sprouting during angiogenesis.

References

Garcia Blanes, M. et al. (2007) J Biol. Chem. 282(14):10660.
Kiselyov, A. et al. (2007) Expert Opin. Investig. Drugs 16(1):83.
Robinson, C.J. & Stringer, S.E. (2001) J. Cell Sci.114:853.
Meyer, M. et al. (1999) EMBO J. 18:363.
Dougher-Vermazen, M. et al. (1994) Bioch Biophys Res Com. 205:728.

Western blot image of GST-recombinant human VEGFR-1 (89 kDa), VEGFR-2 (110 kDa), and VEGFR-3 (86 kDa) C-terminal regions. The blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317), anti-VEGFR-2 (Tyr-801, conserved site), and anti-VEGFR-3 (a.a. 1285-1298).

Left: Western blot image of HUVEC cells untreated (-) or treated with pervanadate (1 mM) for 30 min. (+). Right: Western blot image of GST-recombinant VEGFR-2 kinase without (-) or with (+) akaline phosphatase treatment. Both sets of blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317) or anti-VEGFR-2 (Tyr-801).

Phospho-VEGFR-2 (Tyr-801) peptide (coupled to carrier protein) corresponding to amino acids surrounding Tyr-801 in human VEGFR-2. This sequence has high homology to the conserved site in rat and mouse VEGFR-2, and has significant homology to the conserved sites in VEGFR-1 (Tyr-794) and VEGFR-3 (Tyr-812).

*For more information, see UniProt Accession P35968

Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified with phospho-VEGFR-2 (Tyr-801) peptide. The purified antibody detects a 180 kDa* band corresponding to VEGFR-2 in western blots of human endothelial cells treated with pervanadate, and shows strong reactivity toward recombinant human VEGFR-2. This reactivity is removed by akaline phosphatase treatment. In addition, the antibody detects the conserved site in VEGFR-1 (Tyr-794). The antibody also works for immunofluorescent staining of VEGFR-2 in HUVEC cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Baek, M. et al. (2018) Cell Death Dis. 9(7):730. (WB: mouse keratinocytes)
Chen, P. et al. (2014) Proc Natl Acad Sci. 111(15):5514 (WB: HUVEC cells)
Chen, T.T. et al. (2010) J Cell Biol. 188(4):595. (WB: HUVECs + VEGF)

This kit contains: