Catalog # TP5691

TRPM7 (a.a. 1484-1497) Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:1000
WB	1:1000

Size 100 μl

Species Reactivity Hu

MW 220 kDa

The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. Due to the magnesium inhibition, TRPM7’s ion channel activity is very low. TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. Both Ser-1513 and Ser-1569 phosphorylation is required for kinase activity, and phosphorylation of Ser-1513 may inhibit Caspase-mediated cleavage of the C-terminal tail. Thus, TRPM7 is a multifunctional transmembrane protein with roles in cell signaling, proliferation, migration, and death.

References

Park, HS, et al. (2014) Korean J Phys Pharm. 18(1):15-23.
Desai, BN. et al. (2012) Dev. Cell. 22(6): 1149
Clark, K. et al. (2008) PLoS ONE 3(3): e1876
Masayuki, M. et al. (2005) J of Bio Chem 280(21): 20793

Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) TP5691 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).

TRPM7 (a.a. 1484-1497) synthetic peptide corresponding to amino acid 1484 to 1497 in human TRPM7. This site is not well conserved in rat and mouse TRPM7, and has no homology to other TRPM family members.

*For more information, see UniProt Accession Q96QT4

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody was affinity purified using TRPM7 (a.a. 1484-1497) peptide. This antibody antibody detects a 220 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 cells and detects a 70 kDa recombinant C-terminal kinase domain of human TRPM7.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: