Catalog # SP5531

Sox2 (Lys-119), methyl-specific Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
WB	1:1000
ICC	1:100

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck, F

MW 35 kDa

Embryonic stem cells can maintain a pluripotent state that is controlled by a set of transcription factors that include Oct-4, Sox2, and Nanog. Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development. siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency, while overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram somatic cells to a pluripotent state. Sox2 also regulates adult multipotent progenitors in various epithelial tissues, and may be important for survival and regeneration of these tissues. The activity of Sox2 may be regulated by phosphorylation and methylation. Akt1 phosphorylates Thr-118 and enhances Sox2 transcriptional activity, while Set7 can monomethylate Lys-119 leading to inhibition of Sox2 transcriptional activity, as well as Sox2 ubiquination and degradation. In addition, Sox2 Thr-128 is constituitively phoshorylated in the F9 mouse stem cell line.

References

Fang, L.. et al. (2014) Mol Cell. 55(4):537.
Jeong, C.H. et al. (2010) Stem Cells. 28(12):2141.
Loh, Y.H. et al. (2006) Nat Genet. 38:431.
Boyer, L.A. et al. (2005) Cell. 122:947.

Western blot image of human Sox2 recombinant protein untreated (lanes 1 & 3) or treated with Set7 methyltransferase to methylate Lys-119 (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Lys-119) methyl-specific antibody (lanes 3 & 4).

Immunocytochemical labeling of methylated Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Sox2 (Lys-119) methyl-specific (SP5531). The antibody was detected using goat anti-rabbit DyLight® 594.

Methylated-Sox2 (Lys-119) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Lys-119 in mouse Sox2. This site is well conserved in rat and human Sox2, and has high homology with the conserved site in Sox1 (Lys-127) and Sox3 (Lys-145), but low homology to other Sox family members.

*For more information, see UniProt Accession P48432

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody was cross adsorbed to unmethylated Sox2 (Lys-119) peptide before affinity purification using methylated-Sox2 (Lys-119) peptide. This antibody antibody detects a 34 kDa* protein on SDS-PAGE immunoblots of mouse F9 stem cells, as well as human Sox2 recombinant protein that was methylated by Set7 methyltransferase.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: