Catalog # SP5371

Semaphorin-6A (a.a. 772-787) Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
ICC	1:250
IP	1:100
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck, F

MW 120 kDa

The Semaphorin family of axon guidance molecules includes secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 6A (Sema-6A) is a single-pass type I membrane protein that contains a plexin-semaphorin-integrin (PSI) domain and a Sema domain. Sema-6A is active as a homodimer or oligomer. The Sema-6A homodimer interacts with a Plexin A2 homodimer. Sema-6a promotes reorganization of the actin cytoskeleton to regulate normal granule cell migration in the developing cerebellum and to control axon guidance in the developing central nervous system. In addition, Sema-6a has been implicated in oligodendrocyte myelination and may alter VEGF signaling during angiogenesis.

References

Segarra, M. et al. (2012) Blood. 120(19):4104.
Bernard, F. et al. (2012) Glia. 60(10):1590.
Xu, X.M. (2000) J Neurosci. 20(7):2638.

Western blot of Sema-6A in human colorectal cancer (CRC) cells treated with control (shC) or Sema-6A (shSema6A) shRNAs. Sema-6A was immunoprecipitated from each of the CRC lysates using Sema-6A (a.a. 772-787) or Sema-6A (C-terminus) antibody, then the blotted immunoprecipitations were probed with Sema-6A antibody. (Images provided by Dr. Luca Tamagnone from the IRCC, Univ. of Torino, Italy).

Immunocytochemical labeling of Sema-6A in COS7 cells that were mock transfected (top images) or Sema-6A transfected (bottom images). The cells were labeled with anti-Sema-6A (a.a. 772-787) (Left top and bottom image) or anti-Sema-6A (C-terminus) (Right top and bottom image). The antibodies were detected using anti-rabbit fluorescent secondary antibody. (Images provided by Dr. Luca Tamagnone from the IRCC, University of Torino, Italy).

Semaphorin 6A synthetic peptide (coupled to carrier protein) corresponding to amino acids 772 to 787 in the cytoplasmic region of human Sema-6A. The sequence used has high homology to similar regions in rat and mouse Sema-6A, and has low homology to other semaphorin family members.

*For more information, see UniProt Accession Q9H2E6

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using Semaphorin 6A (a.a. 772-787) peptide (without carrier). The antibody detects a 120kDa* protein corresponding to the apparent molecular mass of Sema-6A on SDS-PAGE immunoblots of Sema-6A transfected COS7 cells, human colorectal cancer cells, and rat PC12 cells. In addition, shRNA knockdown of Sema-6A expression significantly reduces detection of Sema-6A using anti-Sema-6A (a.a. 772-787) antibody.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: