Catalog # SP0381

Sox2 (Thr-128), phospho-specific Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
ICC	1:100
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck, F

MW 35 kDa

Embryonic stem cells can maintain a pluripotent state that is controlled by a set of transcription factors that include Oct-4, Sox2, and Nanog. Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development. siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency, while overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram somatic cells to a pluripotent state. Sox2 also regulates adult multipotent progenitors in various epithelial tissues, and may be important for survival and regeneration of these tissues. The activity of Sox2 may be regulated by phosphorylation and methylation. Akt1 phosphorylates Thr-118 and enhances Sox2 transcriptional activity, while Set7 can monomethylate Lys-119 leading to inhibition of Sox2 transcriptional activity, as well as Sox2 ubiquination and degradation. In addition, Sox2 Thr-128 is constituitively phoshorylated in the F9 mouse stem cell line.

References

Fang, L.. et al. (2014) Mol Cell. 55(4):537.
Jeong, C.H. et al. (2010) Stem Cells. 28(12):2141.
Loh, Y.H. et al. (2006) Nat Genet. 38:431.
Boyer, L.A. et al. (2005) Cell. 122:947.

Western blot of mouse F9 stem cells untreated (lanes 1 & 3) and treated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 antibody (lanes 1 & 2) and rabbit polyclonal Sox2 (Thr-128) phospho-specific antibody (lanes 3 & 4).

Immunocytochemical labeling of phosphorylated Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Sox2 (Thr-128) phospho-specific (SP0381). The antibody was detected using goat anti-rabbit Ig:DyLight® 594.

Phospho-Sox2 (Thr-128) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Thr-128 in mouse Sox2. This site is identical in human Sox2 (Thr-126), and has some homology with the conserved site in mouse Sox1 (Ser-136) and Sox3 (Ser-154).

*For more information, see UniProt Accession P48432

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody was cross adsorbed to unphosphorylated Sox2 (Thr-128) peptide before affinity purification using phospho-Sox2 (Thr-128) peptide. This antibody detects a 34 kDa* protein on SDS-PAGE immunoblots of mouse F9 stem cells. This reactivity is not observed after lambda phosphatase dephosphorylation of Sox2.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: