Catalog # RP2791

Robo1 (C-terminal region) Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
ICC	1:200
WB	1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 180 kDa

The Robo family of repulsive guidance receptors (Robo1-4) have important roles in controlling axon guidance and cell migration. These receptors are members of the immunoglobulin (Ig) superfamily and consist of an ectodomain with five Ig domains and three fibronectin type III repeats, a single transmembrane domain, and a long cytoplasmic tail that contains four blocks of conserved cytoplasmic sequences. In Drosophila, mutations in Robo, and its midline-expressed ligand Slit, result in too many axons crossing and staying at the midline. Several proteins that regulate the actin cytoskeleton, including cAbl, Ena, and Rho-family GTPases, contribute to the Robo signaling pathway. cAbl phosphorylates Robo1 at Tyr-1073, and this may inhibit Robo activity, while Slit-Robo signaling activates both Rac and Rho, and inactivates Cdc42. Thus, Robo guidance receptors control axon outgrowth and cell migration through activation of cell signaling pathways that regulate cytoskeletal dynamics.

References

Yang, L. & Bashaw, G.J. (2006). Neuron 52:595.
Bashaw, G.J. et al. (2000). Cell 101:703.
Kidd, T. et al. (1998) Cell 92:205.

Western blot analysis of HepG2 (lanes 1 & 4), C2C12 (lanes 2 & 5), and HUVEC (lanes 3 & 6). The blot was probed with anti-Robo1 (C-terminal region) in absence (lanes 1-3) or presence of Robo1 (C-terminal region) blocking peptide (RX2795; lanes 4-6).

Immunocytochemical labeling of Robo1 in mouse C2C12. The cells were labeled with rabbit polyclonal Robo1 (C-terminal region) in the absence or presence of blocking peptide (RX2795). The antibody was then detected using appropriate secondary antibodies conjugated to Cy3.

Robo1 synthetic peptide corresponds to amino acids in the C-terminal region of human Robo1 This sequence has significant homology to the same region in rat and mouse Robo1, and has low homology to other Robo family members.

*For more information, see UniProt Accession Q9Y6N7

Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using Robo1 (C-terminal region) peptide. The purified antibody detects a 180 kDa* band corresponding to Robo1 in Western blots of human HepG2 and HUVEC cells, as well as mouse C2C12 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: