Catalog # FM0291

FXYD5/Dysadherin (Extracellular region) M029 Antibody

Mouse Monoclonal

Application / Dilution

ELISA	1:2000
ICC	1:100
IP	1:100
WB	1:1000

Size 100 μl

Species Reactivity Hu

MW 45 kDa

Isotype IgG1

The Na+/K+ ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving Na+ export and K+ import across the plasma membrane. It is composed of a large catalytic α subunit and a membrane-spanning auxiliary β subunit. In humans, the Na+/K+ ATPase is a binary complex of an α subunit that has four isoforms (α1-α4) and a β-subunit that has three isoforms (β1, β2, β3). In addition, the Na+/K+ ATPase can interact with a group of regulatory subunits, the FXYD type I membrane protein family. This family contains FXYD1-7, and each member contains the conserved F-X-Y-D motif in the trans-membrane domain. FXYD5 (Dysadherin) is expressed in a variety of cells and tissue, as well as upregulated in carcinomas. FXYD5 contains extensive O-glycosylation, and is expressed as molecules that range from 35 to 55 kDa depending on cell type. FXYD5 can reduce E-cadherin mediated cell-cell adhesions, and may be involved in carcinogenesis.

References

Lubarski Gotliv I. (2016) Front Cell Dev Biol. 4:26.
Lubarski, I. et al. (2005) J Biol Chem. 280(45):37717.
Ino, Y. et al. (2002) PNAS, U S A. 99(1):365.

Western blot analysis of FXYD5 protein expression. Immunoprecipitates using FM0311 FXYD5 antibody only (lane 1) or FM0311 with A431 lysate (lane 2) or A431 input only (lane 3). Human cell lysates MDA-MB-231 (lane 4) or MeWo (lane 5). The blot was probed with mouse monoclonal FXYD5 (FM0291) at 1:1000.

Immunocytochemical labeling of FXYD5 in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with mouse monoclonal FXYD5 (FM0291). The antibody was detected using goat anti-mouse Ig DyLight® 594.

Clone (M029) was generated from a proprietary antigen related to the native human FXYD5 protein expressed in A431 epidermoid carcinoma cell line.

*For more information, see UniProt Accession Q96DB9

Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M029 mouse monoclonal antibody detects a 35-55 kDa* band on SDS-PAGE immunoblots of human A431, A549, MeWo, MDA-MB-231 cell, as well as human breast, lung, and skin tissues. The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects the FXYD5 protein on live cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: