Catalog # SK6570

Sema-3E and Plexin D1 Receptor Antibody Kit

Antibody Sampler Kit

Application / Dilution

Species Reactivity

Hu, Rt, Ms, Rb, Ck




The Sema-3E and Plexin D1 Receptor kit can be used to monitor the expression of Sema-3E and its receptor Plexin D1. The kit has an N-terminal region Sema-3E antibody, and Plexin D1 antibodies for detecting two regions in the cytoplasmic domain and a region in the extracellular Sema domain. The kit also includes secondary reagent for rabbit polyclonal antibody detection.

Western blot analysis of Plexin D1 expression in human endothelial cells (HUVEC) (lanes 1-6). The blots were probed with rabbit polyclonals anti-Plexin D1 (Sema domain) (lanes 1 & 2), anti-Plexin D1 (Cytoplasmic domain) (lanes 3 & 4), and anti-Plexin D1 (a.a. 1635-1647) (lanes 5 & 6). Each antibody was used in the presence of their respective blocking peptide (lanes 2, 4 & 6).

Western blot analysis of rat PC12 cells (lanes 1 & 2) and human PC-3 cells (lanes 3 & 4). The blots were probed with anti-Sema-3E (N-terminal region) antibody in absence (lanes 1 & 3) or presence of Sema-3E (N-terminal region) blocking peptide (lanes 2 & 4).

Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

Plexin D1 (Cytoplasmic domain) Rabbit pAb50 μlWB, EHu, Rt, Ms250
Plexin D1 (Sema domain) Rabbit pAb50 μlWB, EHu, Rt, Ms250
Plexin D1 (a.a. 1635-1647) Rabbit pAb50 μlWB, EHu, Rt, Ms250
Semaphorin-3E (N-terminal region) Rabbit pAb 50 μlWB, EHu, Rt, Ms,Ck87
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb100 μlWB, E, ICC, IHCRb