Catalog # PP1471

Plexin A1 (N-terminal Sema Domain) Antibody

Rabbit Polyclonal

Application / Dilution

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 220 kDa



Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. This region in Plexin-A1 binds the RhoGTPases, Rnd1 and RhoD. Recruitment of Rnd1 has been implicated in the cytoskeletal collapse that occurs after semaphorin-mediated activation of Plexin-A1, while RhoD may block this collapsing activity through interaction with the cytoplasmic region of Plexin-A1. The expression of Plexin-A1, along with the co-receptor Neuropilin-1, is upregulated in neurons after central nervous system injury. The axons from these neurons cannot cross semaphorin 3A-containing regions at the site of injury. Thus, semaphorin 3A and its co-receptors, Plexin-A1 and Neuropilin-1, may have significant roles in axon regeneration after neuronal injury.


Takahashi, T. & Strittmatter, S.M. (2001) Neuron 29:429.
Tamagnone, L. et al. (1999) Cell 99:71.
Kolodkin, A.L. et al. (1993) Cell 75:1389.

Western blots showing mouse brain (lanes 1 & 4), and Cos-7 cells untransfected (lanes 2 & 5) or transfected with mouse myc-tagged Plexin-A1 (lanes 3 & 6). The blots were probed with either affinity purified anti-Plexin-A1 (PP1471); lanes 1-3) or with mouse monoclonal anti-Myc (lanes 4-6).

Immunocytochemical double labeling using anti-Myc mouse monoclonal and anti-Plexin-A1 rabbit polyclonal (PP1471) antibodies in Cos-7 cells mock transfected (A,D) or transfected with Myc-tagged mouse Plexin-A1 construct (B,E). The specificity of the binding in E was demonstrated by using Plexin-A1 peptide (PX1475) in the presence of this anti-Plexin-A1 antibody (C,F).

Plexin-A1 synthetic peptide (coupled to carrier protein) corresponding to a region within the semaphorin domain in human Plexin-A1. This sequence is highly conserved in rat and mouse Plexin-A1, and has low homology to other Plexin family members.

*For more information, see UniProt Accession Q9UIW2
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 220 kDa* protein corresponding to the apparent molecular mass of Plexin-A1 on SDS-PAGE immunoblots of adult mouse brain and Cos-7 cells transfected with mouse Plexin-A1. In immunocytochemistry, this antibody co-localizes with anti-Myc in Cos-7 cells transfected with Myc-tagged mouse Plexin-A1.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: