Catalog # TL7151
Tubulin (Bovine Brain) Protein
Lysate
| WB | 10 μl/lane |
Size 100 μl
Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of a/β-Tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability.
For preparation of tubulin lysate, bovine brain tubulin was purified and quantified using the BCA method (Pierce) before diluting to the final concentration in gel electrophoresis sample buffer.
Western blot analysis of purified brain tubulin untreated (lanes 1,3,5) or treated with ERK2 kinase to phosphorylate Ser-172 (lanes 2,4,6). The blot was probed with anti-β-Tubulin (a.a. 168-177) (lanes 1 & 2), anti-β-Tubulin (Ser-172) (lanes 3 & 4), and anti-β-Tubulin (TM1541) (lanes 5 & 6).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains:
