Catalog # FL9671

Rabbit Fibroblast Calyculin A Control

Lysate

Application Dilution
WB20 μl/lane

Size 100 μl

DATASHEET  




$75

Calyculin A is a serine/threonine phosphatase inhibitor that inhibits the activity of protein phosphatases PP1 and PP2A. Cells treated with calyculin A for 30 minutes can undergo significant threonine phosphorylation, as shown by western blotting using anti-Phosphoserine/threonine, cat.# PP2551, as compared to untreated, control cell lysates.

Fibroblast from rabbit spleen were harvested to generate a primary fibroblast cell line. Confluent cultures of rabbit fibroblasts were serum starved for 2 hours. Cells were then either left untreated (Cat.# FL9671) or treated with Calyculin A (100 nM) for 30 minutes at 37°C (cat.# FL9681). Cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 1 mM sodium fluoride in 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.



Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY