Sphingosine Kinase 1 + ERK2 Phosphorylated

Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Regulation of SK1 may occur through ERK mediated phosphorylation of Ser-225. This lysate was generated from human recombinant sphingosine kinase 1 that had been phosphorylated by ERK-2 kinase in an in vitro kinase reaction. The lysate can be loaded on mini-gels at 10-20 ml/lane and used as a positive control in western blots probed with anti-sphingosine kinase 1 (SP1621) or anti-sphingosine kinase 1 (Ser-225), phospho-specific (SP1641).

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