Catalog # SL9161

Sphingosine Kinase 1 + ERK2 Phosphorylated

Lysate

Application Dilution
WB10 μl/lane

Size 100 μl

DATASHEET  




$75

Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Regulation of SK1 may occur through ERK mediated phosphorylation of Ser-225. This lysate was generated from human recombinant sphingosine kinase 1 that had been phosphorylated by ERK-2 kinase in an in vitro kinase reaction. The lysate can be loaded on mini-gels at 10-20 ml/lane and used as a positive control in western blots probed with anti-sphingosine kinase 1 (SP1621) or anti-sphingosine kinase 1 (Ser-225), phospho-specific (SP1641).

 



SK1 Lysate is supplied at a concentration of 2 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY