Catalog # SL7461

Sphingosine Kinase 2 Recombinant Protein

Lysate

Application Dilution
WB20 μl/lane

Size 100 μl

DATASHEET  




$75

Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine leading to S1P-mediated cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. Regulation of SK1 and SK2 activity may occur through phosphorylation. SK1 is phosphorylated at Ser-225 by ERK leading to increased activity and translocation to the plasma membrane. SK2 is phosphorylated in response to EGF, PKC activators, and phorbol esters. ERK1 can phosphorylate both Ser-351 and Thr-578, and non-phosphorylatable mutants of these sites suppress ERK1-mediated chemotaxis.

Human recombinant SK2 is a full length protein with an N-terminal GST fusion protein. The protein is detected by rabbit polyclonal anti-SK2 (N-terminal region) antibody (Cat.#SP4621) and anti-SK2 (Thr-578) phospho-specific antibody (Cat.#SP4631).

Western blot of human recombinant SK2 (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-SK2 (N-terminal region) (lanes 1 & 2) or anti-SK2 (Thr-578) (lanes 3 & 4).



SK2 recombinant protein lysate is supplied at a concentration of 2 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY