Catalog # SL7441


Semaphorin-3A Recombinant Protein

Lysate

Application Dilution
WB10 μl/lane

Size 100 μl

$75

One family of inhibitory axon guidance molecules is the semaphorins, which include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance during neuronal development and after CNS injury. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region.

Human recombinant Semaphorin-3A protein includes amino acids 26 to 771, as well as a C-terminal human IgG FC domain. The protein is detected by rabbit polyclonal anti-Sema3A (N-terminal region) (Cat.#SP1401), anti-Sema3A (Central region) (Cat.#SP1221), and anti-Sema3A (C-terminal) (Cat.#SP1241). The antibodies detect both full length Sema3A/Fc chimera (125 kDa) and the cleaved full length Sema3A (95 kDa).

Sema-3A recombinant protein lysate is supplied at a concentration of 10 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blots of neonatal rat brain (lanes 1, 3, & 5) and human recombinant Sema3A/Fc chimera (95/125 kDa) (lanes 2, 4, & 6). Blots were probed with anti-Sema3A (SP1401) (lanes 1 & 2), anti-Sema3A (SP1221) (lanes 3 & 4) and anti-Sema3A (SP1241) (lane 5 & 6).



This kit contains:

KIT SUMMARY