Catalog # SL7171


SYF c-Src-transformed Control

Lysate

Application Dilution
WB20 μl/lane

Size 100 μl

$75

c-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion.

The mouse fibroblast cell line, SYF, is deficient for the c-Src family kinases, c-Src, Yes, and Fyn, while SYF + c-Src overexpress c-Src kinase. This cell line is useful for studies of c-Src mediated phosphorylation when treated with the tyrosine phosphatase inhibitor, pervanadate. Confluent cultures of SYF + c-Src cells were serum starved 2 hrs, and then either left untreated (Cat.# SL7171) or treated with pervanadate (1 mM) for 30 minutes at 37°C (cat.# SL7181). Cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.

Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.



This kit contains:

KIT SUMMARY