Catalog # PL7221

Profilin-1 + PKCα Phosphorylated


Application / Dilution
WB10 μl/lane

Size 100 μl



Lysate Preparation

Profilins are highly conserved, small actin-binding proteins that include Profilin-1 to -4 (PFN1-4). PFN1 is ubiquitously expressed while PFN2 is preferentially enriched in brain. The two testis-specific profilins, PFN3 and PFN4, significantly differ in primary sequence and function compared to PFN1 and PFN2. Profilin is phosphorylated at both tyrosine and serine residues in vivo. Tyr-129 is phosphorylated in response to VEGF-A stimulation, and this promotes profilin actin binding and polymerization. Ser-138 is phosphorylated by ROCK and dephosphorylated by PP1. This serine phosphorylation inhibits G-actin binding, as well as decreases profilin's aggregation suppressor activity by inhibiting binding to huntingtin.

Human recombinant Profilin full length protein (15 kDa) was phosphorylated by PKCα in an in vitro  kinase assay. The phosphorylated Profilin-1 protein is detected by rabbit polyclonal anti-Profilin (Ser-138) phospho-specific (Cat.# PP4791).

Western blot of human recombinant Profilin-1 control (-) or phosphorylated (+) in vitro with PKCα kinase. The blots were probed with anti-Profilin (a.a. 126-137) (left panel) or anti-Profilin (Ser-138) phospho-specific (right panel) antibodies at 1:1000 dilution.

Profilin-1 recombinant protein lysate is supplied at a concentration of 5 ng/μl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: