Catalog # PL7121

PC12 (undifferentiated)

Lysate

Application Dilution
WB20 μl/lane

Size 100 μl

DATASHEET  




$75

Rat pheochromocytoma-derived PC12 cells are an important cell model for studies of growth factor cell signaling pathways. PC12 has been used for a variety of studies where cell division and cell differentaiton have been induced through growth factor activation. Both NGF and FGF can promote neurite proliferation, while EGF and IGF-1 can induce mitosis in PC12 cells. In addition, PC12 cells can be used as a model of neuron development since they differentiate into a neuron-like morphology and express various neuronal proteins.

PC12 cells were differentiated into neurite-bearing cells by plating the cells on rat tail collagen coated dishes and growing the cells in the presence of NGF (200 ng/ml) for 48 hrs (Cat.# PL7141). Undifferentiated PC12 cells (PL7121) were grown in suspension cultures. Both PC12 cell models were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 1 mM sodium fluoride, 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.

PC12 cells grown on rat tail collagen were undifferentiated (Control) or differentiated (200 ng/ml NGF) for 48 hrs. The cells were then fixed and labeled for immunocytochemistry using anti-βIII-Tubulin (C-terminus; TP1691).



Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY