Catalog # PF7501

Phalloidin:FITC

Reagent

Application / Dilution

ICC	1:500
IHC	1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck, F

Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F-actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin.
Fluorescent conjugates of phalloidin are commonly used to label actin filaments for immunocytochemical and immunohistochemical applications. Both rhodamine and fluorescein labeled phalloidin can be used to localize and quantify actin filaments in cells and tissues.

References

Wulf, E. et al. (1979) Proc. Nat. Acad. of Sci.(USA). 76:4498.
Faulstich, H. et al. (1983) Exp. Cell Res. 144:73.
Faulstich, H. et al. (1988) J. Muscle Res. Cell Motility. 9:370.

Fluorescent labeling of actin filaments in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The fixed cells were labeled with phalloidin:FITC (PF7501) at a dilution of 1:500.

Phalloidin conjugated to FITC via the side chain of amino acid 7. The conjugate has a molecular weight of 1250. This fluorochrome has an excitation maximum of 495 nm and emission maximum of 513 nm.

Phalloidin conjugates are supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Barnes J et al. (2018) Mol Autism. 9:44. (ICC: human stem cells)
Kline, A. et al. (2018) Dev Biol. 440(2):99. (ICC: Drosophila ovary)

This kit contains: