Catalog # PF7551

Phalloidin:TRITC

Reagent

Application / Dilution

ICC	1:1000
IHC	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck, F

Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F-actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin.
Fluorescent conjugates of phalloidin are commonly used to label actin filaments for immunocytochemical and immunohistochemical applications. Both rhodamine and fluorescein labeled phalloidin can be used to localize and quantify actin filaments in cells and tissues.

References

Wulf, E. et al. (1979) Proc. Nat. Acad. of Sci.(USA). 76:4498.
Faulstich, H. et al. (1983) Exp. Cell Res. 144:73.
Faulstich, H. et al. (1988) J. Muscle Res. Cell Motility. 9:370.

Fluorescent labeling of actin filaments in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The fixed cells were labeled with phalloidin:TRITC (PF7551) at a dilution of 1:2000.

Phalloidin conjugated to TRITC via the side chain of amino acid 7. The conjugate has a molecular weight of 1300. This fluorochrome has an excitation maximum of 540 nm and emission maximum of 570 nm.

Phalloidin conjugates are supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Errede, M. et al. (2018) Fluids Barriers CNS. 15:28. (ICC: human brain pericytes)
Quan, H. et al. (2018) J Biomed Mater Res A. 106(10):2625. (ICC: mouse macrophages)
Kline, A. et al. (2018) Dev Biol. 440(2):99. (ICC: Drosophila ovary)
Bade, ND et al. (2018) Biophys J. 114(6):1467. (ICC: mouse embryonic fibroblast)
Sahoo, N. et al. (2017) Dev Cell. 41(3):262. (ICC: mouse parietal cells)
Marín, T, et al. (2014) Nutrients 6(8): 3000 (IF: cAble/p73 mouse cerebellum)
Vargas, M. et al. (2014) PLoS ONE 9(3): e92309 (ICC: actin in dendritic spines)

This kit contains: