Catalog # OK7670


Organelle Marker

Immunocytochemistry Kit

$455

A variety of subcellular compartments in eukaryotic cells perform specific activities related to cell function. The nucleus encloses DNA containing chromatin where gene transcriptions occurs. Endoplasmic reticulum, Golgi, and endosomes are involved in protein transport and protein post-translational modification and folding. Mitochondria contain proteins involved in cellular respiration, apoptosis, and free radical production. Caveolae are abundant cell-surface organelles involved in lipid regulation and endocytosis. Immunocytochemical co-labeling of newly discovered proteins with organelle marker antibodies is an important technique for determining protein function.

References
Chevet, E. et al. (2010) Semin Cell Dev Biol. 21(5):486.
Bungard, D. et al. (2010) Science. 329(5996):1201.
Lau, A.T. et al. (2011) J Biol Chem. 286(30):26628.
Grundtman, C. et al. (2011) Arterioscler Thromb Vasc Biol. 31(5):960.
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

GM3421 Martín-Acebes, M.A. et al. (2014) J virology 88(20): 12041. Data not shown
HP4291 Seo, Y. et al. (2013) PLoS ONE 8(8):e75005. WB: MDCK, A549, and HEK293
HP4291 Soe, K.C. et al. (2013) BBA 1829(11):1184. WB: Sf9 cells
GM3421 Martın-Acebes, M.A. et al. (2011) PLoS ONE 6(9): e24970. ICC: monkey Vero cell

Immunocytochemical labeling of calnexin (CM4371) (top left), Hsp60 (HM4381) (top middle), EEA1 (EM3471) (top right), GM130 (GM3421) (bottom left), histone H2B (HP4291) (bottom middle), and caveolin-1 (CP2781) (bottom right). The antibodies were detected using secondary antibodies conjugated to DyLight® 488 or DyLight® 594.



This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYICC DILUTION
Calnexin (N-terminal region) Mouse mAb50 μlWB, E, ICCHu, Rt, Ms1:50
Caveolin-1 (N-terminal region) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck1:100
Early Endosome Antigen (EEA1) Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms1:50
GM130 (C-terminal region) Mouse mAb50 μlWB, E, ICCHu, Rt, Ms1:50
Histone H2B (C-terminus)Rabbit pAb 50 μlWB, E, ICCHu, Rt, Ms, F1:200
Hsp60 (N-terminal region) Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms1:100
KIT SUMMARY

The organelle market kit can be used to co-localize proteins to specific organelles in cells. The kit includes a panel of antibodies that detect the following cell organelles:

Caveolae = Caveolin 1
Endoplasmic Reticulum = Calnexin
Endosomes = EEA1
Golgi = GM130
Mitochondria = Hsp60
Nucleus = Histone H2B