Catalog # ML9621

Monocyte (PMA-differentiated)

Lysate

Application Dilution
WB20 μl/lane

Size 100 μl

DATASHEET  




$75

PMA treatment of THP-1 monocytic leukemia cells is a common strategy for studies of mature monocyte function in vitro. Confluent cultures of THP-1 monocytic cells were either left untreated (Cat.# ML9611) or treated with PMA (1 μM) for 72 hrs at 37°C (cat.# ML9621). Differentiated cells become adherent monocyte-like cells that exhibit many characteristics of mature monocytes. After 72 hrs, the cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 1 mM sodium fluoride in 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.

 



Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY