PMA treatment of THP-1 monocytic leukemia cells is a common strategy for studies of mature monocyte function in vitro. Confluent cultures of THP-1 monocytic cells were either left untreated (Cat.# ML9611) or treated with PMA (1 μM) for 72 hrs at 37°C (cat.# ML9621). Differentiated cells become adherent monocyte-like cells that exhibit many characteristics of mature monocytes. After 72 hrs, the cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 1 mM sodium fluoride in 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains: