Catalog # LL8061

LIMK1 (activated) + λ phosphatase Dephosphorylated

Lysate

Application Dilution
WB10 μl/lane

Size 100 μl

DATASHEET  




$75

LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains. LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK. PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr-508 or Thr-505 residues in the activation loop, increasing LIMK activity. In addition, VEGF-induced stress fiber formation has been linked to p38-mediated activation of LIMK through MK-2 phosphorylation of Ser-323. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser-3 residue of cofilin.

An activated His-tagged mouse LIMK1 contains Thr-508 phosphorylation. The phosphorylated kinase is detected by anti-LIMK1 (N-terminus) Cat.# LP1831 and anti-LIMK1 (Thr-508) Cat.# LP1891. This activated kinase can be dephosphorylated at Thr-508 by treating with l-phosphatase for 30 min. at 37°C.



LIMK (activated) + lambda phosphatase Lysate is supplied at a concentration of 2 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY