Catalog # KL8201

K-562 Pervanadate Control

Lysate

Application Dilution
WB20 μl/lane

Size 100 μl

DATASHEET  




$75

K-562 lysate was derived from a human chronic myelogenous leukemia (CML) cell line. Pervanadate is a protein tyrosine phosphatase inhibitor that is commonly used to increase tyrosine phosphorylation in cells. When cells are treated with pervanadate for 30 minutes they undergo significant tyrosine phosphorylation.

Confluent cultures of K-562 cells were serum starved for 2 hours. Cells were then either left untreated (Cat.# KL8201) or treated with pervanadate (1 mM) for 30 minutes at 37°C (cat.# KL8301). Cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.

Western blot analysis of K-562 control (lane 1) and pervanadate treated (lane 2) cells (20 µg/lane). The blot was probed with anti-Paxillin mouse monoclonal Cat. # PM1071.



Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY