Catalog # JL9541


Jurkat Camptothecin-induced Apoptosis

Lysate

Application Dilution
WB20 μl/lane

Size 100 μl

$75

The camptothecin treatment of human Jurkat T-Cell leukemia cells can be used as a western blot positive control for detecting antigens expressed during apoptosis. The treated cells undergo apoptosis as determined by identification of specific apoptotic cleavage products, such as PARP and caspase-3 cleavage.

Confluent cultures of Jurkat cells were either left untreated (Cat.# JL9531) or treated with Camptothecin (4 μM) for 4 hours at 37°C (cat.# JL9541). Cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 1 mM sodium fluoride in 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.

Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.



This kit contains:

KIT SUMMARY