The camptothecin treatment of human Jurkat T-Cell leukemia cells can be used as a western blot positive control for detecting antigens expressed during apoptosis. The treated cells undergo apoptosis as determined by identification of specific apoptotic cleavage products, such as PARP and caspase-3 cleavage.
Confluent cultures of Jurkat cells were either left untreated (Cat.# JL9531) or treated with Camptothecin (4 μM) for 4 hours at 37°C (cat.# JL9541). Cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 1 mM sodium fluoride in 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains: