Catalog # HL7361

Histone H2B Recombinant Protein

Lysate

Application Dilution
WB10 μl/lane

Size 100 μl

DATASHEET  




$75

The nucleosome is a protein complex consisting of four core histones (H2A, H2B, H3, and H4). Two molecules of each histone forms an octamer that makes up the nucleosome. DNA wraps around repeating nucleosome units to generate chromatin structures. The structure of chromatin determines the accessiblity to transcription factors. Post-translational modification of the amino-terminal tail of histones in nucleosomes alters chromatin structure to promote or inhibit transcription. Histone H2B activity is regulated through phosphorylation at multiple sites Ser-14, Ser-32, and Ser-36 in the amino-terminal region. AMPK phosphorylates Ser-36 on histone H2B during cell stress leading to increased transcription and cell survival, while ectopic expression of an unphosphorylatable histone H2B during cell stress reduces transcription of AMPK-dependent genes and lowers cell survival.

Human recombinant Histone H2B is a full length protein (15 kDa) expressed in E. Coli. The protein is detected by rabbit polyclonal anti-Histone H2B (C-terminus) (Cat.#HP4291) and anti-Histone H2B (a.a. 33-47) (Cat.#HP4311).



Histone H2B recombinant protein lysate is supplied at a concentration of 10 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY