Catalog # EL7301

Human Endothelial + Pervanadate Treated

Lysate

Application Dilution
WB20 μl/lane

Size 100 μl

DATASHEET  




$75

Pervanadate is a protein tyrosine phosphatase inhibitor that is commonly used to increase tyrosine phosphorylation in cells. Human umbilical vein endothelial cells treated with pervanadate for 30 minutes display a significant increase in tyrosine phosphorylation as shown by western blotting using anti-Phosphotyrosine.

Confluent cultures of human umbilical cord endothelial cells were serum starved 2 hrs at 37°C. Cells were then either left untreated (Cat.# EL7201) or treated with pervanadate (1 mM) for 30 minutes at 37°C (Cat.# EL7301). Cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.

Western blot analysis of control and pervanadate treated human endothelial cells (20 µg/lane). The blot was probed with anti-Phosphotyrosine rabbit polyclonal.



Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY